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| Status |
Public on Aug 11, 2010 |
| Title |
Whole blood transcriptional profiles of patients with active tuberculosis (TB) and other inflammatory and infectious diseases |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Tuberculosis (TB), caused by infection with Mycobacterium tuberculosis (M. tuberculosis), is a major cause of morbidity and mortality worldwide and efforts to control TB are hampered by difficulties with diagnosis, prevention and treatment. Most people infected with M. tuberculosis remain asymptomatic, termed latent TB, with a 10% lifetime risk of developing active TB disease, but current tests cannot identify which individuals will develop disease. The immune response to M. tuberculosis is complex and incompletely characterized, hindering development of new diagnostics, therapies and vaccines. The goals of this study include: 1. Identify a transcript signature for active TB in intermediate and high burden settings, correlating with radiological extent of disease and reverting to that of healthy controls following treatment; 2. Identify a specific transcript signature that discriminated active TB from other inflammatory and infectious diseases; 3. Classify TB signature using modular and pathway analysis tools.
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| Overall design |
Three milliliters of whole blood was collected in Tempus tubes from 12 pediatric streptococcus, 40 pediatric staphylococcus, 31 still’s disease, 82 pediatric systemic lupus erythematosus (SLE) and 28 adult SLE patients. RNA was extracted and globin reduced. Labeled cRNA was hybridized to Illumina Human HT-12 Beadchips. Healthy controls were included to match patients’ demographic data. Genespring software was used to analyze active TB transcript signatures, comparing with healthy controls and other inflammatory and infectious diseases.
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| Citation(s) |
20725040 |
| |
| Submission date |
Jun 02, 2010 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Damien Chaussabel |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor Institute for Immunology Research
|
| Street address |
3434 Live Oak
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75204 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (274)
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| This SubSeries is part of SuperSeries: |
| GSE19491 |
Blood Transcriptional Profiles in Human Active and Latent Tuberculosis |
|
| Relations |
| BioProject |
PRJNA128917 |
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