|
| Status |
Public on Sep 29, 2015 |
| Title |
Molecular analysis of normal oviduct, STIC and invasive serous cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
High-grade serous cancer (HGSC) progresses to advanced stages without symptoms and the 5-year survival rate is a dismal 30%. Recent studies of ovaries and fallopian tubes in patients with BRCA mutations revealed that pre-metastatic HGSC is found almost exclusively in the fallopian tube in a lesion termed “serous tubal intraepithelial carcinoma” or STIC. We have performed laser captured microdissection (LCM) of normal oviduct, STIC and invasive serous cancer from each patient. A whole-genome transcriptome analysis comparing between normal oviduct, STIC and invasive serous cancer were performed. We demonstrated a clear molecular progression from normal to STIC, which shared the gene expression patterns with invasive serous cancer, suggesting a new set of genes as basis of novel detection and therapeutic approaches to HGSC at its earliest stage.
|
| |
|
| Overall design |
Uisng frozen sections, LCM was performed to collect normal oviduct, STIC and invasive serous cancer regions from one patient. Samples were collected from 6 independent patiens. Total RNA was isolated with Picopure RNA isolation kit. We used affymetrix HuEx-1_0-st-v2 array to hybridize samples from 6 patients.
|
| |
|
| Contributor(s) |
Yamamoto Y, Ning G, Howitt B, Wu L, McKeon F, Crum CP, Xian W |
| Citation(s) |
26415052 |
| |
| Submission date |
Jun 01, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Frank McKeon |
| E-mail(s) |
[email protected]
|
| Phone |
8608372486
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3455 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA285475 |
Less...
More...