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Series GSE21

Query DataSets for GSE21

Status

Public on Feb 12, 2002

Title

snf/swi mutants of S. cerevisiae.

Organism

Saccharomyces cerevisiae

Experiment type

Expression profiling by array

Summary

The Saccharomyces cerevisiae Snf/Swi complex has been previously demonstrated to control transcription and chromatin structure of particular genes in vivo and to remodel nucleosomes in vitro. We have performed whole-genome expression analysis, using DNA microarrays, to study mutants deleted for a gene encoding one conserved (Snf2) or one unconserved (Swi1) Snf/Swi component. This analysis was performed on cells grown in both rich and minimal media. The microarray results, combined with Northern blot, computational, and genetic analyses, show that snf2Delta and swi1Delta mutations cause similar effects on mRNA levels, that Snf/Swi controls some genes differently in rich and minimal media, and that Snf/Swi control is exerted at the level of individual genes rather than over larger chromosomal domains. In addition, this work shows that Snf/Swi controls mRNA levels of MATalpha-specific genes, likely via controlling transcription of the regulators MATalpha1 and MCM1. Finally, we provide evidence that Snf/Swi acts both as an activator and as a repressor of transcription, and that neither mode of control is an indirect effect of the other.
This study is described in more detail in Sudarsanam P et al.(2000) Proc Natl Acad Sci U S A 97:3364-9
Keywords: other

Contributor(s)

Sudarsanam P, Iyer VR, Brown PO, Winston F

Citation(s)

10725359

Submission date

Feb 08, 2002

Last update date

Jan 18, 2013

Organization

Stanford Microarray Database (SMD)

E-mail(s)

[email protected]