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| Status |
Public on Jan 01, 2005 |
| Title |
Seed development in LEAFY COTYLEDON1 mutants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of gene expression in Ws-0 lec1 (LEAFY COTYLEDON1) mutant Arabidopsis thaliana. Developmental stages studied includes 24-Hr post-fertilization, globular stage, cotyledon stage, mature green stage, post-mature green stage, and seedlings.
Microarray Methods: Total RNA was extracted using the Hot Borate Method [Stones et. al. PNAS vol 98 no 20: 11806-11811 (2001)]. Biotinylated cRNA were prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit (Farmingdale, NY). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 4.0 (MAS 4.0) with a global scaling intensity set at 500.
Keywords: other
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| Contributor(s) |
Le BH, Bui AQ, Pelletier J, Kwong LW, Okamuro JK, Fischer RL, Drews GN, Harada JJ, Goldberg RB |
| Citation(s) |
28739919 |
| |
| Submission date |
Feb 17, 2004 |
| Last update date |
Jun 27, 2019 |
| Contact name |
Bob Goldberg |
| E-mail(s) |
[email protected]
|
| Phone |
310-825-3270
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| Organization name |
University of California, Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
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| Street address |
610 Charles E Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (2) |
| GPL71 |
[AG] Affymetrix Arabidopsis Genome Array |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA87127 |
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