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Status
Public on Jan 01, 2005
Title
WT Mid-Maturation Seed 1 (AtGenome1)
Sample type
RNA
Source name
Genotype: Ws-0, Wild Type; Stage: Mid-Maturation Seed; Technical Replica: 1 of 2
Organism
Arabidopsis thaliana
Extracted molecule
total RNA
Description
Microarray Methods: Total RNA was extracted using the Hot Borate Method (ref.). Fifteen micrograms of biotinylated complementary RNA (cRNA) was prepared as described (Le et. al., 2004, PNAS). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 4.0 (MAS 4.0) with a global scaling intensity set at 2500.
Submission date
Feb 17, 2004
Last update date
May 28, 2005
Contact name
Bob Goldberg
E-mail(s)
[email protected]
Phone
310-825-3270
Organization name
University of California, Los Angeles
Department
Molecular, Cell and Developmental Biology
Street address
610 Charles E Young Drive East
City
Los Angeles
State/province
CA
ZIP/Postal code
90095
Country
USA
Platform ID
GPL71
Series (2)
GSE680
Transcript Profiling of Arabidopsis Plant Life Cycle
GSE1051
Seed development in LEAFY COTYLEDON1 mutants
Data table header descriptions
ID_REF
VALUE
AVG_DIFF - This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL
A qualitative measurement indicating if the probe set is detected (Present ; P), not detected (Absent; A), or marginally detected (Marginal; M)
Data table
ID_REF
VALUE
ABS_CALL
AFFX-MurIL2_at
-346.3
A
AFFX-MurIL10_at
-243.9
A
AFFX-MurIL4_at
-75.8
A
AFFX-MurFAS_at
-53.3
A
AFFX-BioB-5_at
2547.9
P
AFFX-BioB-M_at
2798.3
P
AFFX-BioB-3_at
2597.2
P
AFFX-BioC-5_at
6590.8
P
AFFX-BioC-3_at
6532.5
P
AFFX-BioDn-5_at
9080.4
P
AFFX-BioDn-3_at
28516
P
AFFX-CreX-5_at
61634.7
P
AFFX-CreX-3_at
78858.7
P
AFFX-BioB-5_st
-261.3
A
AFFX-BioB-M_st
-24.6
A
AFFX-BioB-3_st
-86
A
AFFX-BioC-5_st
-470.9
A
AFFX-BioC-3_st
-268.1
A
AFFX-BioDn-5_st
419.8
A
AFFX-BioDn-3_st
193.6
A
Total number of rows: 8297 Table truncated, full table size 143 Kbytes .Supplementary file
Size
Download
File type/resource
GSM16996.CEL.gz
1.7 Mb
(ftp) (http)
CEL