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Sample GSM10478

Query DataSets for GSM10478

Status

Public on Mar 31, 2005

Title

lec1-1 24-Hr Seed 2

Sample type

RNA

Source name

Genotype: Ws-0, lec1-1; Stage: 24-Hr Fertilized Seed; Biological Replica: 2 of 2

Organism

Arabidopsis thaliana

Extracted molecule

total RNA

Description

Growth Condition: Plants were grown in a Conviron chamber under continuous light with fluorescent lamps at 20oC and 50% - 70% relative humidity. Experiments were performed as described (Le et. al., 2003, PNAS).

Microarray Methods: Total RNA was extracted using the Hot Borate Method (ref.). Fifteen micrograms of biotinylated complementary RNA (cRNA) was prepared as described (Le et. al., 2003, PNAS). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 5.0 (MAS 5.0) with a global scaling intensity set at 500.
Keywords = Arabidopsis
Keywords = seed development

Submission date

Sep 25, 2003

Last update date

Aug 28, 2018

Contact name

Bob Goldberg

E-mail(s)

[email protected]

Phone

310-825-3270

Organization name

University of California, Los Angeles

Department

Molecular, Cell and Developmental Biology

Street address

610 Charles E Young Drive East

City

Los Angeles

State/province

ZIP/Postal code

90095

Country

USA

Platform ID

GPL198

Series (1)

GSE1051

Seed development in LEAFY COTYLEDON1 mutants

Relations

Reanalyzed by

GSE119083

Data table header descriptions
ID_REF
VALUE	This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL	A qualitative measurement indicating if the probe set is detected (Present ; P), not detected (Absent; A), or marginally detected (Marginal; M)
Detection p-value	A p-value indicating the significance of the Detection call

Data table
ID_REF	VALUE	ABS_CALL	Detection p-value
AFFX-BioB-5_at	886.3	P	0.00006
AFFX-BioB-M_at	1454.9	P	0.00006
AFFX-BioB-3_at	1179.5	P	0.000052
AFFX-BioC-5_at	1758.1	P	0.00007
AFFX-BioC-3_at	1192.9	P	0.000044
AFFX-BioDn-5_at	2103.1	P	0.000044
AFFX-BioDn-3_at	8082.2	P	0.000044
AFFX-CreX-5_at	18261.5	P	0.000044
AFFX-CreX-3_at	25884.4	P	0.000044
AFFX-DapX-5_at	6.8	A	0.382599
AFFX-DapX-M_at	5.6	A	0.941556
AFFX-DapX-3_at	4.4	A	0.953518
AFFX-LysX-5_at	35.2	A	0.354453
AFFX-LysX-M_at	12.1	A	0.891021
AFFX-LysX-3_at	5.7	A	0.659339
AFFX-PheX-5_at	11.4	A	0.843268
AFFX-PheX-M_at	31.3	A	0.659339
AFFX-PheX-3_at	39.3	A	0.712257
AFFX-ThrX-5_at	10.2	A	0.843268
AFFX-ThrX-M_at	2.2	A	0.989683

Total number of rows: 22810

Table truncated, full table size 588 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM10478.CEL.gz	3.2 Mb	(ftp)(http)	CEL