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Status
Public on Jan 01, 2005
Title
WT 24-Hr Seed 1 (AtGenome1)
Sample type
RNA
Source name
Genotype: Ws-0, Wild Type; Stage: 24-Hr fertilized seed; Technical Replica: 1 of 2
Organism
Arabidopsis thaliana Extracted molecule
total RNA
Description
Microarray Methods: Total RNA was extracted using the Hot Borate Method (ref.). Fifteen micrograms of biotinylated complementary RNA (cRNA) was prepared as described (Le et. al., 2004, PNAS). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 4.0 (MAS 4.0) with a global scaling intensity set at 2500.
Submission date
Feb 17, 2004
Last update date
May 28, 2005
Contact name
Bob Goldberg
E-mail(s)
[email protected] Phone
310-825-3270
Organization name
University of California, Los Angeles
Department
Molecular, Cell and Developmental Biology
Street address
610 Charles E Young Drive East
City
Los Angeles
State/province
CA
ZIP/Postal code
90095
Country
USA
Platform ID
GPL71 Series (2)
GSE680 Transcript Profiling of Arabidopsis Plant Life Cycle
GSE1051 Seed development in LEAFY COTYLEDON1 mutants
Data table header descriptions ID_REF
VALUE AVG_DIFF - This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL A qualitative measurement indicating if the probe set is detected (Present ; P), not detected (Absent; A), or marginally detected (Marginal; M)
Data table ID_REF VALUE ABS_CALL AFFX-MurIL2_at
-101.1
A
AFFX-MurIL10_at
-5.8
A
AFFX-MurIL4_at
66.7
A
AFFX-MurFAS_at
116.7
A
AFFX-BioB-5_at
2035.3
P
AFFX-BioB-M_at
2948.7
P
AFFX-BioB-3_at
3040.1
P
AFFX-BioC-5_at
6255.7
P
AFFX-BioC-3_at
6342
P
AFFX-BioDn-5_at
10180.5
P
AFFX-BioDn-3_at
23924.7
P
AFFX-CreX-5_at
55941.2
P
AFFX-CreX-3_at
69536.9
P
AFFX-BioB-5_st
102.7
A
AFFX-BioB-M_st
255.4
A
AFFX-BioB-3_st
-185.1
A
AFFX-BioC-5_st
-305.5
A
AFFX-BioC-3_st
-146.2
A
AFFX-BioDn-5_st
309.7
A
AFFX-BioDn-3_st
164.8
A
8297 143 Kbytes .Supplementary file Size Download File type/resource GSM17007.CEL.gz
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