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Sample GSM10458

Query DataSets for GSM10458

Status

Public on Jan 01, 2005

Title

WT Seedling 2

Sample type

RNA

Source name

Genotype: Ws-0, Wild Type; Stage: 3-Day-After-Imbibition Seedling; Biological Replica: 2 of 2

Organism

Arabidopsis thaliana

Extracted molecule

total RNA

Description

Growth Condition: Plants were grown in a Conviron chamber under continuous light with fluorescent lamps at 20oC and 50% - 70% relative humidity. Experiments were performed as described (Le et. al., 2003, PNAS).

Microarray Methods: Total RNA was extracted using the Hot Borate Method (ref.). Fifteen micrograms of biotinylated complementary RNA (cRNA) was prepared as described (Le et. al., 2003, PNAS). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 5.0 (MAS 5.0) with a global scaling intensity set at 500.
Keywords = Arabidopsis
Keywords = seed development

Submission date

Sep 24, 2003

Last update date

Aug 28, 2018

Contact name

Bob Goldberg

E-mail(s)

[email protected]

Phone

310-825-3270

Organization name

University of California, Los Angeles

Department

Molecular, Cell and Developmental Biology

Street address

610 Charles E Young Drive East

City

Los Angeles

State/province

ZIP/Postal code

90095

Country

USA

Platform ID

GPL198

Series (2)

GSE680	Transcript Profiling of Arabidopsis Plant Life Cycle
GSE1051	Seed development in LEAFY COTYLEDON1 mutants

Relations

Reanalyzed by

GSE119083

Data table header descriptions
ID_REF
VALUE	This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL	A qualitative measurement indicating if the probe set is detected (Present ; P), not detected (Absent; A), or marginally detected (Marginal; M)
DETECTION P-VALUE	A p-value indicating the significance of the Detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	487.3	P	0.000857
AFFX-BioB-M_at	939.8	P	0.00007
AFFX-BioB-3_at	736.4	P	0.00007
AFFX-BioC-5_at	1434.9	P	0.000095
AFFX-BioC-3_at	795.8	P	0.000052
AFFX-BioDn-5_at	1354.1	P	0.000044
AFFX-BioDn-3_at	6241.8	P	0.000044
AFFX-CreX-5_at	11156.4	P	0.000044
AFFX-CreX-3_at	14828.5	P	0.000044
AFFX-DapX-5_at	18	A	0.165861
AFFX-DapX-M_at	26.8	A	0.250796
AFFX-DapX-3_at	1.7	A	0.998898
AFFX-LysX-5_at	23.4	A	0.262827
AFFX-LysX-M_at	5.8	A	0.960339
AFFX-LysX-3_at	11.6	A	0.617401
AFFX-PheX-5_at	17.4	A	0.760937
AFFX-PheX-M_at	2.2	A	0.989683
AFFX-PheX-3_at	7.3	A	0.852061
AFFX-ThrX-5_at	4.5	A	0.921998
AFFX-ThrX-M_at	32.2	A	0.60308

Total number of rows: 22810

Table truncated, full table size 588 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM10458.CEL.gz	3.3 Mb	(ftp)(http)	CEL