|
| Status |
Public on Dec 08, 2017 |
| Title |
The histone code reader Spin1 controls skeletal muscle development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report developmental defects in Spin1(Myf5-Cre) mice, in which Spin1 is ablated in skeletal muscle.
|
| |
|
| Overall design |
RNA sequencing of skeletal muscle of Spin1(Myf5-Cre) and control fetuses at E15.5 and E16.5 and of tibialis anterior of surviving Spin1(Myf5-Cre) and control mice at three weeks of age (P21).
ChIP sequencing using primary myoblast isolated from 10-day old mice and anti-SPIN1 antibody (custom SPIN1-5867) or anti-H3K4me antibody (Diagenode, C15410003).
|
| |
|
| Contributor(s) |
Greschik H, Willmann D, Schüle R |
| Citation(s) |
29168801 |
| |
| Submission date |
Dec 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Holger Greschik |
| E-mail(s) |
[email protected]
|
| Organization name |
University Medical Center Freiburg
|
| Department |
ZKF
|
| Lab |
Prof. Roland Schüle
|
| Street address |
Breisacher Strasse 66
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (25)
|
|
| Relations |
| BioProject |
PRJNA357994 |
| SRA |
SRP095320 |
Less...
More...