|
| Status |
Public on Dec 08, 2017 |
| Title |
PriMBs_H3K4me3 (ChIP-seq) |
| Sample type |
SRA |
| |
|
| Source name |
skeletal muscle
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: primary myoblasts
age: isolated from 10-day-old mice
chip antibody: anti-H3K4me antibody (Diagenode, catalog# C15410003, lot# A5051-001P)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Primary myoblasts (PriMBs) were isolated from limb muscle of 10-day old mice using the preplating technique and cultivated to about 70% confluency. Chromatin was prepared and ChIP performed according to Arrigoni, L. et al. (2016) Nucleic Acids Res. 55, e67.
Libraries were prepared using the NEBNext Ultra DNA library preparation kit (NEB, E7370S). Adapter-ligated fragments were amplified with 12 PCR cycles and sequenced on the Illumina HiSeq 2500 platform.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Paired-end reads were mapped to the mouse reference genome (mm10) using bowtie 2.
Data were further analyzed using the peak finding algorithm of MACS 1.4.2 using input as control.
Genome_build: mm10
Supplementary_files_format_and_content: bed, wig
|
| |
|
| Submission date |
Apr 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Holger Greschik |
| E-mail(s) |
[email protected]
|
| Organization name |
University Medical Center Freiburg
|
| Department |
ZKF
|
| Lab |
Prof. Roland Schüle
|
| Street address |
Breisacher Strasse 66
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE92539 |
The histone code reader Spin1 controls skeletal muscle development |
|
| Relations |
| BioSample |
SAMN06680363 |
| SRA |
SRX2704850 |
