|
| Status |
Public on Dec 08, 2017 |
| Title |
E15.5_KO_rep2 (RNA-seq) |
| Sample type |
SRA |
| |
|
| Source name |
skeletal muscle
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: skeletal muscle
age: E15.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA (according to the manufacturer's instructions) from skeletal muscle of front and hind limb of fetuses at E15.5 and E16.5, and from tibialis anterior of mice at three weeks of age (P21).
RNA samples were sequenced by the standard Illumina protocol to create raw sequence files (.fastq).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Reads were aligned to the mm10 build of the mouse genome using TopHat version 2.
Aligned reads were counted with the Homer software (analyzeRepeats) and differentially expressed genes identified using edgeR.
Genome_build: mm10
Supplementary_files_format_and_content: Excel tables containing normalized read counts obtained from edgeR
|
| |
|
| Submission date |
Dec 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Holger Greschik |
| E-mail(s) |
[email protected]
|
| Organization name |
University Medical Center Freiburg
|
| Department |
ZKF
|
| Lab |
Prof. Roland Schüle
|
| Street address |
Breisacher Strasse 66
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE92539 |
The histone code reader Spin1 controls skeletal muscle development |
|
| Relations |
| BioSample |
SAMN06163242 |
| SRA |
SRX2435152 |
