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| Status |
Public on Sep 22, 2007 |
| Title |
IL-1 treatment of human epidermal keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1a. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal transduction, proliferation, and epidermal differentiation. IL-1 induces many genes that have antimicrobial function. Secreted cytokines, chemokines, growth factors, and their receptors are the prominent targets of IL-1 regulation, including IL-8, IL-19, elafin, C3, and S100A proteins, which implicates IL-1 in the pathogenesis of inflammatory diseases. IL-1 induced not only proliferation-associated genes but also differentiation marker genes such as transglutaminase-1 and involucrin, which suggests that IL-1 plays an important role in the aberrant proliferation and differentiation seen in psoriasis. Correlation of IL-1 regulated genes with the TNFa and IFNg regulated ones showed more similarities between IL-1 and TNFa than IL-1 and IFNg, whereas Oncostatin-M affected a largely unrelated set of genes. IL-1 regulates many genes previously shown to be specifically over-expressed in psoriasis. In summary, IL-1 regulates a characteristic set of genes that define its specific contribution to inflammation and aberrant differentiation in skin diseases.
Keywords: Time course
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| Overall design |
keratinocytes were treated with 25 ng/ml IL-1 and transcriptomes compared to untreated controls at 1, 4, 24 and 48 h post treatment.
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| Contributor(s) |
Blumenberg M, Yano S, Banno T, Walsh R |
| Citation(s) |
17941080 |
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| Submission date |
Sep 20, 2007 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Miroslav Blumenberg |
| E-mail(s) |
[email protected]
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| Phone |
212 263-5924
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| Organization name |
NYU School of Medicine
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| Department |
Dermatology
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| Lab |
Blumenberg
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| Street address |
455 First Ave #874
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (8)
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| GSM230879 |
Human epidermal keratinocytes treated with IL-1 1hr |
| GSM230880 |
Human epidermal keratinocytes controls 1 hr |
| GSM230881 |
Human epidermal keratinocytes control 4 hr |
| GSM230882 |
Human epidermal keratinocytes treated with IL-1 for 4 hr |
| GSM230883 |
Human epidermal keratinocytes control 24 h |
| GSM230884 |
Human epidermal keratinocytes treated with IL-1 for 24 hr |
| GSM230913 |
Human epidermal keratinocytes treated controls 48 hr |
| GSM230914 |
Human epidermal keratinocytes treated with IL-1 for 48 hr |
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| Relations |
| BioProject |
PRJNA102655 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9120_RAW.tar |
16.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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