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| Status |
Public on Oct 30, 2015 |
| Title |
Effects of Salidroside on gene expression in ischemic brain of rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We employed DNA microarray to identify genes differentially regulated by salidroside in ischemic brain of MCAO rats. With a cut of p < .01 and 2-fold change, we found that 121 genes were upregulated and 142 genes were down-regulated in the MCAO group, compared to the sham group. Administration of salidroside (1 h after reperfusion, daily for 6 d) enhanced 28 transcripts and suppressed 16 transcripts. The genes involved in neuroplasticity and oxygen carrier were further confirmed by qRT-PCR. Our data suggest that salidroside could regulate neuroplasticity in post-ischemic stroke, in addition to its neuroportective function.
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| Overall design |
MCAO rats were injected intraperitoneally with salidroside (50 mg/kg body weight) or vehicle (saline) once a day for 6 d, commencing 1 h after reperfusion (n=3 /group). Ischemic brain was dissected for RNA extraction. Differential gene expression regulated by salidroside were analysed by microarray and subsequently confirmed by qRT-PCR.
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| Contributor(s) |
Hong G, Lai W, Zhang X |
| Citation(s) |
25911293 |
| |
| Submission date |
Nov 01, 2013 |
| Last update date |
Mar 11, 2019 |
| Contact name |
guizhu HONG |
| E-mail(s) |
[email protected]
|
| Phone |
00447886936531
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| Organization name |
Fujian University of Traditional Chinese Medicine
|
| Department |
Centre of Biomedical Research & Development
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| Street address |
No. 1 Huatou Road, Minhou Shangjie
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| City |
Fuzhou, |
| State/province |
Fujian |
| ZIP/Postal code |
350122 |
| Country |
China |
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| Platforms (1) |
| GPL14746 |
Agilent-028282 Whole Rat Genome Microarray 4x44K v3 (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA226085 |
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