|
| Status |
Public on Oct 30, 2015 |
| Title |
[B] |
| Sample type |
RNA |
| |
|
| Source name |
sham brain 2
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: male
tissue: brain
|
| Treatment protocol |
MCAO rats were injected intraperitoneally with salidroside (50 mg/kg body weight) or vehicle (saline) once a day for 6 d, commencing 1 h after reperfusion (n=3 /group)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ischemic brain was dissected for RNA extraction with an RNeasy Mini Kit, in accordance with the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA concentration and quality were determined by NanoDrop ND-1000 (NanoDrop Technologgies, Wilmington, DE) and RNA gel electrophoresis assays, respectively.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from each sample was amplified linearly and labeled with Cy3-UTP. The labeled cRNAs were purified with an RNeasy Mini Kit (Qiagen). Concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.
|
| |
|
| Hybridization protocol |
Purified and labeled cRNA was hybridized to Rat 4x44K Gene Expression Array (Agilent Technologies), according to the manufacturer’s procedures
|
| Scan protocol |
The hybridized arrays were washed, fixed, and scanned using the Agilent DNA Microarray Scanner (Agilent Technologies).Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images.
|
| Description |
gene expression in sham control
|
| Data processing |
Normalization and subsequent data processing were performed with the GeneSpring GX v12.0 software package (Agilent Technologies). After normalization of the raw data, genes that were detected in all samples were chosen for further data analysis
|
| |
|
| Submission date |
Nov 01, 2013 |
| Last update date |
Oct 30, 2015 |
| Contact name |
guizhu HONG |
| E-mail(s) |
[email protected]
|
| Phone |
00447886936531
|
| Organization name |
Fujian University of Traditional Chinese Medicine
|
| Department |
Centre of Biomedical Research & Development
|
| Street address |
No. 1 Huatou Road, Minhou Shangjie
|
| City |
Fuzhou, |
| State/province |
Fujian |
| ZIP/Postal code |
350122 |
| Country |
China |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE52001 |
Effects of Salidroside on gene expression in ischemic brain of rats |
|
