GEO DataSets

(Submitter supplied) No studies systematically compared time-resolved and multi-tissue innate immune responses for European eels (Anguilla anguilla) with Aeromonas hydrophila infection through a high throughput method. Here, we challenged European eels with A. hydrophila (infected) or PBS (control). A low fatality rate (16.1%) was observed for infected group at 2 days post-infection (dpi). Then we examined transcriptional profiles of intestines, livers and spleens from 12 European eels with/without the infection of A. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24544

36 Samples

Download data: FA, TXT

(Submitter supplied) Since the identification of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the main maturation-inducing hormone (MIH) in Japanese eel A. japonica, female European eels have been injected with this steroid to induce oocyte maturation and ovulation. However, the females injected with DHP need to ovulate rapidly following the DHP injection to release good quality eggs. In this study, we compared treatment of progesterone (P) with DHP treatment as control, in vitro, to determine dose-response effects, and in vivo, at a single dose. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28518

12 Samples

Download data: XLSX

(Submitter supplied) Vibrio vulnificus is a marine zoonotic pathogen associated with fish farms that is considered a biomarker of climate change. Zoonotic strains trigger a rapid death of their susceptible hosts (fish or humans) by septicemia that has been linked to a cytokine storm in mice. A toxin called RtxA1 produced by the bacteria might play an important role in bacterial invasion and subsequent death by septic shock since animals infected with a mutant deficient in rtxA1 suffer from septicemia but do not die. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL16775

63 Samples

Download data: TXT

(Submitter supplied) We investigated transcriptional and translational changes in rete mirabile tissue of European yellow and silver eels. We therefore analyzed the transcriptome as well as the proteome of rete capillaries of specimens of both developmental stages. The results clearly show the presence of a large number of membrane transport proteins in the transcriptome as well as in the proteome. ATP required to energize ion and metabolite transport can be generated in the aerobic metabolism. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20640 GPL28518

11 Samples

Download data: FA, XLSX

(Submitter supplied) In spite of many decades of research, the spawning migration of the European eel Anguilla anguilla from the European coast to the Sargasso Sea remains a mystery. In particular, the role of the swimbladder as a buoyancy regulating structure is not yet understood. In this study, we exercised silver eels in a swim tunnel under elevated hydrostatic pressure. The transcriptome of gas gland tissue of these exercised eels was then compared to the known transcriptome of not exercised (control) silver eel gas gland cells. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20640 GPL28518

19 Samples

Download data: XLSX

(Submitter supplied) Nanometric revolution is underway, promising technical innovations in a wide range of applications, leading to a potential boost in environmental discharges. Nanoparticle propensity to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary consumers while a lack of knowledge for higher trophic levels persists. This study focused on a predatory fish, the European eel Anguilla anguilla exposed to gold nanoparticles (AuNP, 10 nm, PEG-coated) for 21 days at three concentration levels in food: 0 (NP0), 1 (NP1) and 10 (NP10) mg Au.kg-1 . more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24571

24 Samples

Download data: CSV, FASTA

(Submitter supplied) This project aims to study the role played by small non-coding RNAs in the response of aquatic organisms to the presence of micropollutants in the environment. MiRNA were purified from Eels (Anguilla anguilla) sampled from two sites along the Gironde aquatic system with contrasted pollution profiles.

Organism:

Anguilla anguilla

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24544

12 Samples

Download data: CSV

(Submitter supplied) We investigated transcriptional changes caused by the nematode Anguillicola crassus within yellow and silver eels by comparing gas gland tissues of uninfected yellow with infected yellow eels, and uninfected silver with infected silver eels, respectively. In yellow eel gas gland, the infection caused a modification of steady state mRNA levels of 1675 genes, most of them being upregulated. Functional annotation analysis based on GO terms was used to categorize identified genes with regard to swimbladder metabolism or response to the infection. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20640

26 Samples

Download data: XLSX

(Submitter supplied) Dietary immunostimulants are considered as an effective tool for enhancing the immune status of cultured organisms and improving their general welfare. Immunostimulants are chemical substances which activate transiently elements of the immune response and may potentially render animals more resistant to infectious diseases. They also reduce the risk of disease outbreaks, if administrated prior to situations known to result in stress and impaired general performance (e.g. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL18071

21 Samples

Download data: TXT

(Submitter supplied) Ecotoxicogenomic studies face the problem of the lack of gene sequence information available for toxicologically relevant non-model pollution sentinel species. In this sense, next generation sequencing technologies allow obtaining deep de novo sequence information cost-effectively. We have thus employed the platform GS-FLX of Roche technologies, in order to sequence the multitissue transcriptome of a fish species under severe population decline, the European eel (Anguilla anguilla), using normalized cDNA (Evrogen). more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL18071

30 Samples

Download data: TXT

(Submitter supplied) Many enigmas surround different aspects of freshwater eel biology and life cycle. In the same way different hypothesis about why eels are disappearing from European continental waters have been proposed. One such proposal defends that poor fat accumulation of eels, due to pollution in continental waters, might be stopping eels from reproductive migration to the Sargasso Sea. Thus, habitat deterioration could be blamed for the decline in the catchment potential of the European rivers. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL18071

66 Samples

Download data: TXT

(Submitter supplied) Gene expression analyses have been performed on liver tissue of sexually mature and immature males using microarrays. 60 eels were transferred to two independent temperature controlled recirculation water units connected to two 500 L cylindro-conical tanks (30 fish per tank) where the fish were acclimated to seawater (35 PSU salinity) over a 2 week period.Eel males in one of the seawater units were injected intramuscularly every week over a 140 day period with 2000 IU hCG/kg (human chorionic gonadotropin, Sigma–Aldrich Chemical) dissolved in 0.9% saline to induce sexual maturation. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

8 Samples

Download data: TXT

(Submitter supplied) The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. Three-year-old farmed silver female European eels were injected with 20 mg salmon pituitary extract (SPE) once per week and sampled after 4 weekly hormone injections. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20640

6 Samples

Download data: XLSX

(Submitter supplied) The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. To find leads for maturation markers we performed a pilot deep-sequencing transcriptome analysis of ovarian tissue derived from a yellow eel, a prepubertal silver eel and a post-spawning matured eel. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20639

3 Samples

Download data: XLSX

(Submitter supplied) Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the explanations of the collapse of this species. Among their possible effects, pollutants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. more...

Organism:

Anguilla anguilla; Anguilla rostrata

Type:

Expression profiling by array

Platform:

GPL19017

15 Samples

Download data: TXT

(Submitter supplied) Aquatic ecosystems are subjected to a variety of man-induced stressors but also vary spatially and temporally due to variation in natural factors. In such complex environments, it remains difficult for ecotoxicologists to evaluate the effects of contaminants in wild organisms. The aim of the present study was to test the possibility to detect and unravel the effects of different anthropogenic and natural factors on wild fish by the use of a DNA microarray. more...

Organism:

Anguilla rostrata; Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL19017

84 Samples

Download data: TXT

(Submitter supplied) Our main objectives were: 1) to test whether the caudal fin may be used to detect the effects of pollutant exposure by means of DNA microarray; 2) to test whether the fin may be used to detect the effects of pollutants in wild contaminated fish; 3) to investigate the mechanisms of toxicity for Cd metal. Detecting and unraveling specific effects of contaminants in a multi-stress field context remain a major challenge in ecotoxicology. more...

Organism:

Anguilla rostrata; Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL19017

45 Samples

Download data: TXT

(Submitter supplied) Gene expression analyses have been performed on brain, liver and muscle of eels (Anguilla anguilla) sampled in three different segment of the Canal des Etangs ( artificial canal linking Arcachon Basin and Lacanau Lake). The present study relies on intraspecific differences of glass eels encountered below and above the water dams. Eels were collected using electric fishing under similar climatic and hydrological conditions. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

24 Samples

Download data: TXT

(Submitter supplied) Gene expression analyses have been performed on brain tissue of sexually mature and immature males using microarrays. 60 eels were transferred to two independent temperature controlled recirculation water units connected to two 500 L cylindro-conical tanks (30 fish per tank) where the fish were acclimated to seawater (35 PSU salinity) over a 2 week period.Eel males in one of the seawater units were injected intramuscularly every week over a 140 day period with 2000 IU hCG/kg (human chorionic gonadotropin, Sigma–Aldrich Chemical) dissolved in 0.9% saline to induce sexual maturation. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

8 Samples

Download data: TXT

(Submitter supplied) An European eel-specific microarray platform was developed to identify genes involved in response to pollutants. A comparative analysis of gene expression was conducted between European eel Anguilla anguilla individuals from lowly-polluted Wijmeers pond at Uitbergen (Belgium), highly-polluted Hazewinkel pond at Willebroek (Belgium), extremely-polluted Dessel-Schotel canal at the locations of Schotel (Belgium) and low polluted Bolsena lake (Italy) environments.

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

38 Samples

Download data: TXT