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| Status |
Public on Sep 14, 2020 |
| Title |
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
| Organism |
Anguilla anguilla |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In spite of many decades of research, the spawning migration of the European eel Anguilla anguilla from the European coast to the Sargasso Sea remains a mystery. In particular, the role of the swimbladder as a buoyancy regulating structure is not yet understood. In this study, we exercised silver eels in a swim tunnel under elevated hydrostatic pressure. The transcriptome of gas gland tissue of these exercised eels was then compared to the known transcriptome of not exercised (control) silver eel gas gland cells. Due to the high infection rate of the eel population with the swimbladder parasite Anguillicola crassus, the comparison also included an exercised group of silver eels with a heavily damaged swimbladder, and we compared the previously published transcriptome of not exercised silver eels with a highly damaged swimbladder with the exercised group of silver eels with a heavily damaged swimbladder. The comparisons of unexercised (control) silver eels with exercised silver eels with functional swimbladder (EF), as well as with exercised silver eels with damaged swimbladder(ED), both showed a significant elevation in transcripts related to glycolytic enzymes. This could also be observed within the comparison of unexercised silver eels with a highly infected swimbladder with exercised eels with a damaged swimbladder (DED). In contrast to EF, in ED a significant elevation in transcript numbers of mitochondrial NADH dehydrogenase was observed. While in EF the transcriptional changes suggested that acid production and secretion was enhanced, in ED these changes appeared to be related to thickened tissue and thus elevated diffusion distances. The remarkable number of differentially expressed transcripts coding for proteins connected to cAMP-dependent signaling pathways indicated that metabolic control in gas gland cells includes cAMP-dependent pathways. In contrast to ED, in EF significant transcriptional changes could be related to the reconstruction of the extracellular matrix, while in ED tissue repair and inflammation was more pronounced. Surprisingly, in exercised eels hypoxia inducible transcription factor expression was elevated. In EF, a large number of genes related to the circadian clock were transcriptionally modified, which may be connected to the circadian vertical migrations observed during the spawning migration.
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| Overall design |
Using Illumina Sequencing to obtain RNA-Seq data and statistical Deseq analysis, the transcriptomes of European eel gas gland tissues of animals with different previous swimming exercise and with different gas gland tissue health status were compared.
Please note that the control samples in GSE102221 were re-analyzed in the current study and the duplicated sample records were included for the convenient retrieval of the complete raw data from SRA (as indicated in the sample description field).
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| Contributor(s) |
Schneebauer G, Lindemann C, Drechsel V, Marohn L, Wysujack K, Santidrian E, Dirks RP, Hanel R, Pelster B |
| Citation(s) |
32997701 |
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| Submission date |
May 11, 2020 |
| Last update date |
Oct 05, 2020 |
| Contact name |
Gabriel Schneebauer |
| Organization name |
University of Innsbruck
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| Street address |
Technikerstrasse 25
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| City |
Innsbruck |
| ZIP/Postal code |
6020 |
| Country |
Austria |
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| Platforms (2) |
| GPL20640 |
Illumina HiSeq 2500 (Anguilla anguilla) |
| GPL28518 |
Illumina NovaSeq 6000 (Anguilla anguilla) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA631790 |
| SRA |
SRP261172 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE150340_DESeq_IL-14-12_vs_FG1817.xlsx |
14.4 Mb |
(ftp)(http) |
XLSX |
| GSE150340_DESeq_IL-14-12_vs_FG1924.xlsx |
14.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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