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| Status |
Public on Nov 01, 2024 |
| Title |
Time-resolved and multi-tissue RNAseq provided new insights on the immune responses of European eels following infection with Aeromonas hydrophila |
| Organism |
Anguilla anguilla |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
No studies systematically compared time-resolved and multi-tissue innate immune responses for European eels (Anguilla anguilla) with Aeromonas hydrophila infection through a high throughput method. Here, we challenged European eels with A. hydrophila (infected) or PBS (control). A low fatality rate (16.1%) was observed for infected group at 2 days post-infection (dpi). Then we examined transcriptional profiles of intestines, livers and spleens from 12 European eels with/without the infection of A. hydrophila at 6, 18 and 36 hours post-infection (hpi). The results showed that the most differentially expressed genes (DEGs) were found in the spleens at 6 hpi (7569 DEGs), followed by the intestines at 6 hpi (3129 DEGs) and the livers at 18 hpi (2722 DEGs). Only a few DEGs were found in three tissues at 36 hpi. The comparisons for DEGs numbers and KEGG enrichments suggested a consistent time-course immune responses among these three tissues. A similar enrichment of PRRs-related pathways including TLRs, NLRs and RLRs was revealed in the livers and spleens, but not in the intestines. Among immune-related DEGs, 62 paralogues pairs were found, and the expression trends of most paralogues pairs were consistent. Some paralogues of immune-related DEGs had unique domains. Furthermore, a larger clusters representing the similar tissue were revealed for the intestines and spleens. The distinct cytokine signal transduction and interaction existed between the livers and spleens. Altogether, the present study provides time-resolved and multi-tissue transcriptome characteristics of A. anguilla in response to A. hydrophila infection.
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| Overall design |
we challenged European eels with A. hydrophila (infected) or PBS (control). A low fatality rate (16.1%) was observed for infected group at 2 days post-infection (dpi). Then we examined transcriptional profiles of intestines, livers and spleens from 12 European eels with/without the infection of A. hydrophila at 6, 18 and 36 hours post-infection (hpi).
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| Contributor(s) |
Xiong F, Cao L, Xiong J, Wu YF, Huang WS, Chang MX |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 10, 2021 |
| Last update date |
Nov 01, 2024 |
| Contact name |
cao lu |
| E-mail(s) |
[email protected]
|
| Phone |
+8618202736768
|
| Organization name |
Institute of Hydrobiology
|
| Department |
Chinese Academy of Sciences
|
| Street address |
7 Donghu South Road
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430072 |
| Country |
China |
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| Platforms (1) |
| GPL24544 |
Illumina HiSeq 4000 (Anguilla anguilla) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA779398 |
| SRA |
SRP345424 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE188550_I18PBS1_I18PBS2_vs_I18Ah1_I18Ah2.txt.gz |
35.7 Kb |
(ftp)(http) |
TXT |
| GSE188550_I36PBS1_I36PBS2_vs_I36Ah1_I36Ah2.txt.gz |
2.1 Kb |
(ftp)(http) |
TXT |
| GSE188550_I6PBS1_I6PBS2_vs_I6Ah1_I6Ah2.txt.gz |
33.4 Kb |
(ftp)(http) |
TXT |
| GSE188550_L18PBS1_L18PBS2_vs_L18Ah1_L18Ah2.txt.gz |
40.9 Kb |
(ftp)(http) |
TXT |
| GSE188550_L36PBS1_L36PBS2_vs_L36Ah1_L36Ah2.txt.gz |
724 b |
(ftp)(http) |
TXT |
| GSE188550_L6PBS1_L6PBS2_vs_L6Ah1_L6Ah2.txt.gz |
11.2 Kb |
(ftp)(http) |
TXT |
| GSE188550_RAW.tar |
22.0 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE188550_Sp18PBS1_Sp18PBS2_vs_Sp18Ah1_Sp18Ah2.txt.gz |
30.0 Kb |
(ftp)(http) |
TXT |
| GSE188550_Sp36PBS1_Sp36PBS2_vs_Sp36Ah1_Sp36Ah2.txt.gz |
8.6 Kb |
(ftp)(http) |
TXT |
| GSE188550_Sp6PBS1_Sp6PBS2_vs_Sp6Ah1_Sp6Ah2.txt.gz |
91.0 Kb |
(ftp)(http) |
TXT |
| GSE188550_the_sequences_for_all_IDs.fa.gz |
31.0 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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