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| Status |
Public on Jan 01, 2015 |
| Title |
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation |
| Platform organisms |
Anguilla anguilla; Anguilla rostrata |
| Sample organism |
Anguilla anguilla |
| Experiment type |
Expression profiling by array
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| Summary |
Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the explanations of the collapse of this species. Among their possible effects, pollutants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. The aim of this study was to investigate the potential effect of pollution on the gonad development of wild female silver eels. Female silver eels from two sites with differing contamination levels were artificially matured. Transcriptomic analyses by means of a 1000 candidate gene cDNA microarray were performed on gonads after 11 weeks of maturation. The results showed that the transcription levels of several genes that were associated to the gonadosomatic index (GSI) were involved in mitotic cell division but also in spermatogenesis. Genes associated to pollution were mainly involved in the mechanisms of protection against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated fish.
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| Overall design |
Comparison between wild european eels from a clean (Certes) and contaminated site (Gironde) were performed using a common reference (named in raw data reference, colored with cyanine 5) on microarray.
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| Contributor(s) |
Baillon L, Pierron F, Oses J, Bureau du Colombier S, Caron A, Normandeau E, Lambert P, Couture P, Labadie P, Budzinski H, Dufour S, Bernatchez L, Baudrimont M |
| Citation missing |
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| Submission date |
Dec 17, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
lucie baillon |
| Organization name |
université de Bordeaux UMR ePOC
|
| Street address |
place du docteur Peyneau
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| City |
Arcachon |
| ZIP/Postal code |
33120 |
| Country |
France |
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| Platforms (1) |
| GPL19017 |
American and European eel microarray |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA270623 |
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