GEO DataSets

(Submitter supplied) We characterized transcriptomes of Sinorhizobium meliloti root nodule bacteria with mutations in sigma factor genes.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

20 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

65 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes for strains overexpressing each of the Sinorhizobium meliloti ECF sigma factors the via a plasmid-borne, melibiose-inducible promoter plasmid (PmelA; pCAP11: Pinedo et al. 2008 J Bacteriol 190:2947-2956) compared to control strains carrying the empty vector.

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

45 Samples

Download data: CEL

(Submitter supplied) We characterized the transcriptome of a Sinorhizobium meliloti emmA insertion mutant strain.

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) For transcript analysis of responses in Medicago truncatula to its symbiont Sinorhizobium meliloti wild type or the succinoglycan-deficient exoY mutant we compared transcripts from line A17 roots inoculated with 25 mL OD = 0.05 S. meliloti wild type or exoY mutant. Keywords: 1 line; 2 S. meliloti samples

Organism:

Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4799

6 Samples

Download data

(Submitter supplied) Sinorhizobium meliloti lives as a soil saprophyte, and engages in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including 11 extracytoplasmic function (ECF) sigmas. more...

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) Investigation of whole genome gene expression level changes in a Sinorhizobium meliloti 1021 rpoH1 rpoH2 double mutant, compared to the wild-type strain. The mutations engineered into this strain render it deficient in symbiotic nitrogen fixation. The mutants analyzed in this study are further described in Mitsui, H, T. Sato, Y. Sato, and K. Minamisawa. 2004. Sinorhizobium meliloti RpoH1 is required for effective nitrogen-fixing symbiosis with alfalfa. more...

Organism:

Sinorhizobium meliloti 1021

Type:

Expression profiling by array

Platform:

GPL19375

20 Samples

Download data: CALLS, PAIR

(Submitter supplied) Salt stress is a major agricultural concern inhibiting not only plant growth but also the symbiotic association between legume roots and the soil bacteria rhizobia. This symbiotic association is initiated by a molecular dialogue between the two partners, leading to the activation of a signaling cascade in the legume host and ultimately the formation of nitrogen-fixing root nodules. Here we show that a moderate salt stress increases the responsiveness of early symbiotic genes in Medicago truncatula to its symbiotic partner, Sinorhizobium meliloti, while conversely, inoculation with S. more...

Organism:

Medicago truncatula

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23899

12 Samples

Download data: TXT

(Submitter supplied) PhyR is an unusual type of response regulator consisting of a receiver domain and an extracytoplasmic function (ECF) sigma factor-like domain. It was recently described as a master regulator of general stress response in Methylobacterium extorquens. Orthologues of this regulator are present in essentially all free-living Alphaproteobacteria. In most of them, phyR is genetically closely linked to a gene encoding an ECF sigma factor. more...

Organism:

Bradyrhizobium japonicum; Bradyrhizobium diazoefficiens USDA 110

Type:

Expression profiling by array

Platform:

GPL3401

18 Samples

Download data: CEL, CHP

(Submitter supplied) The dual genome Affymetrix SymbiosisChip contains probe sets for S. meliloti ORFs annotated in 2001, intergenic regions over 150 bp, and ~10,000 probe sets corresponding to expressed sequence tags of the plant host, Medicago truncatula. Protocol: see manufacturer's website

Organism:

Sinorhizobium meliloti; Medicago truncatula

29 Series

275 Samples

Download data: CDF, TXT

(Submitter supplied) Symbiotic nitrogen fixation (SNF) is an energetically expensive process performed by bacteria known as rhizobia during endosymbiotic relationships with leguminous plants. The bacteria require the plant to provide a carbon source for generation of the reductant to power SNF. Although it is well known that C4-dicarboxylates (succinate, fumarate, malate) function as the primary, if not sole, carbon source provided to the rhizobia, the relative contribution of each C4-dicarboxylate is not known. more...

Organism:

Pisum sativum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23779

6 Samples

Download data: XLSX

(Submitter supplied) The RNA-binding protein Hfq is a global regulator, which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) Within this work we identified and characterized SMc03169 (hhrA) as a new Sinorhizobium meliloti gene product with relevance to biological nitrogen fixation symbiosis with leguminous plants. HhrA belongs to the TetR-family of repressors and its deletion from S. meliloti genome affected considerably gene expression as well as several phenotypic traits.

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

6 Samples

Download data: CEL

(Submitter supplied) Sinorhizobium meliloti can live as a soil saprophyte, and can engage in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including two putative RpoH (heat shock) sigmas. more...

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

27 Samples

Download data: CEL

(Submitter supplied) We wanted to test the effect on global gene expression of depleting the essential cell cycle regulator CtrA in order to determine the genes both indirectly and directly transcriptionally regulated by CtrA Gene expression changes in S. meliloti 1,2,4 and 6 hours post CtrA depletion

Organism:

Sinorhizobium meliloti; Sinorhizobium meliloti 1021

Type:

Expression profiling by array

Platform:

GPL18182

24 Samples

Download data: TXT

(Submitter supplied) Sinorhizobium meliloti establishes symbiotic relationship with compatible leguminous plants by inducing root nodule formation, colonizing such nodules, and fixing molecular nitrogen for the host in exchange for carbon compounds. This mutualistic process requires complex communication and tight regulation to allow yet constrain infection to specific tissues. Production of succinoglycan, or exopolysaccharide-I (EPS-I), enables S. more...

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

9 Samples

Download data: CEL, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

24 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes of a strain overexpressing syrM. Our work shows that the syrM transcriptome shares similar gene expression changes to the syrA and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. more...

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes of a strain overexpressing syrA. Our work shows that the syrA transcriptome shares similar gene expression changes to the syrM and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. more...

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes of strains containing a K214T mutation in chvI, with and without overexpression of syrA. Our work shows that overexpression of syrA suppresses many of the transcription changes observed in the chvI K214T mutant.

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

12 Samples

Download data: CEL