|
| Status |
Public on Jun 01, 2010 |
| Title |
Expression data from wildtype Sinorhizobium meliloti Rm1021 and an hfq mutant |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Sinorhizobium meliloti |
| Experiment type |
Expression profiling by array
|
| Summary |
The RNA-binding protein Hfq is a global regulator, which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture.
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| Overall design |
Gene expression profiling of Sinorhizobium meliloti Rm1021 or its isogenic hfq deletion mutant, grown to late exponential phase in rich medium, was performed using custom Affymetrix GeneChips.
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| Contributor(s) |
Barnett MJ, Long SR, Gao M, Teplitski M |
| Citation(s) |
20192823 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM093628 |
Control of Symbiotic Gene Expression in Sinorhizobium meliloti |
STANFORD UNIVERSITY |
SHARON R LONG |
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| |
| Submission date |
Dec 07, 2009 |
| Last update date |
Feb 17, 2015 |
| Contact name |
Melanie J Barnett |
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Sharon R. Long
|
| Street address |
371 Jane Stanford Way
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
|
| Samples (6)
|
| GSM480458 |
Sinorhizobium meliloti hfq deletion mutant, biological rep1 |
| GSM480459 |
Sinorhizobium meliloti hfq deletion mutant, biological rep2 |
| GSM480460 |
Sinorhizobium meliloti hfq deletion mutant, biological rep3 |
| GSM480461 |
Sinorhizobium meliloti Rm1021, biological rep1 |
| GSM480462 |
Sinorhizobium meliloti Rm1021, biological rep2 |
| GSM480463 |
Sinorhizobium meliloti Rm1021, biological rep3 |
|
| Relations |
| BioProject |
PRJNA121725 |
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