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| Status |
Public on Mar 31, 2015 |
| Title |
The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrM) |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Sinorhizobium meliloti |
| Experiment type |
Expression profiling by array
|
| Summary |
We characterized transcriptomes of a strain overexpressing syrM. Our work shows that the syrM transcriptome shares similar gene expression changes to the syrA and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression.
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| Overall design |
Gene expression profiling of a Sinorhizobium meliloti nodD1, nodD2, nodD3 mutant overexpressing syrM was performed using custom Affymetrix GeneChips
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| Contributor(s) |
Barnett MJ, Long SR |
| Citation(s) |
25777671 |
| |
| Submission date |
Sep 18, 2014 |
| Last update date |
Jun 30, 2015 |
| Contact name |
Melanie J Barnett |
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Sharon R. Long
|
| Street address |
371 Jane Stanford Way
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
|
| Samples (6)
|
| GSM1507432 |
Sinorhizobium meliloti A2102 pTE3, biological rep1 |
| GSM1507433 |
Sinorhizobium meliloti A2102 pTE3, biological rep2 |
| GSM1507434 |
Sinorhizobium meliloti A2102 pTE3, biological rep3 |
| GSM1507435 |
Sinorhizobium meliloti A2102 pS73, biological rep1 |
| GSM1507436 |
Sinorhizobium meliloti A2102 pS73, biological rep2 |
| GSM1507437 |
Sinorhizobium meliloti A2102 pS73, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE61526 |
The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 |
|
| Relations |
| BioProject |
PRJNA261386 |
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