|
| Status |
Public on Oct 30, 2024 |
| Title |
Hippocampus_J20_WT [204027420043_R04C01] |
| Sample type |
genomic |
| |
|
| Source name |
Hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hippocampus
externalsampleid: J20_C3
Sex: F
dob: 3/17/2015
dod: 3/15/2016
agedays: 364
mousestrain: C57BL6
ad_model: J20
genotype: WT
|
| Treatment protocol |
Mice were terminally anaesthetized with pentobarbital (intraperitoneal injection) and transcardially perfused with phosphate-buffered saline (PBS). The entorhinal cortex was dissected from the left brain hemisphere on wet ice and snap-frozen on dry ice for subsequent analysis
|
| Growth protocol |
Animals were housed under standard conditions (constant temperature and humidity) with a 12h light/dark cycle in individually ventilated cages, with free access to food (Teklad irradiated global rodent diet (Envigo, United Kingdom)) and water.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA from all samples was isolated from the entorhinal cortex or hippocampus using the AllPrep DNA/RNA Mini Kit (Qiagen)
|
| Label |
Cy3, Cy5
|
| Label protocol |
DNA methylation was measured using the the Mammalian Methylation Array (HorvathMammalMethylChip4, GEO: GPL28271)
|
| |
|
| Hybridization protocol |
Not provided
|
| Scan protocol |
Illumina HiScan
|
| Data processing |
Normalized Average Beta calculated using the SeSame package in R
|
| |
|
| Submission date |
Oct 30, 2023 |
| Last update date |
Oct 30, 2024 |
| Contact name |
Emma Walker |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Exeter
|
| Street address |
RILD Barrack Rd
|
| City |
Exeter |
| ZIP/Postal code |
EX2 5DW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL28271 |
| Series (2) |
| GSE246559 |
Methylomic signatures of tau and beta amyloid in transgenic mouse models of Alzheimer’s disease neuropathology [HorvathMammalMethylChip40 Methylation Beadchip] |
| GSE246561 |
Methylomic signatures of tau and beta amyloid in transgenic mouse models of Alzheimer’s disease neuropathology |
|
