|
| Status |
Public on Sep 30, 2011 |
| Title |
Subject 4 after LCD |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
after LCD
|
| Organism |
Homo sapiens |
| Characteristics |
source material: study participant, subcutaneous adipose tissue, periumbilical area
weight class: weight regainer
time: after LCD
|
| Treatment protocol |
Subcutaneous adipose tissue samples from the periumbilical area were obtained by needle aspiration under local anaesthesia after an overnight fast at each of the time points. Biopsy samples were stored at -80°C until analysis.
|
| Growth protocol |
8 week low calorie diet (LCD; 3.3 MJ/d; 800 kcal).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy total RNA Mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
According to Agilent instructions. RNA from study participant labeled with Cy5, RNA from reference pool labeled with Cy3.
|
| |
|
| Channel 2 |
| Source name |
reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
source material: commercial pool of human liver, heart, intestine tissue, skeletal muscle RNA
|
| Treatment protocol |
Subcutaneous adipose tissue samples from the periumbilical area were obtained by needle aspiration under local anaesthesia after an overnight fast at each of the time points. Biopsy samples were stored at -80°C until analysis.
|
| Growth protocol |
8 week low calorie diet (LCD; 3.3 MJ/d; 800 kcal).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy total RNA Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
According to Agilent instructions. RNA from study participant labeled with Cy5, RNA from reference pool labeled with Cy3.
|
| |
|
| |
| Hybridization protocol |
Sample preparation, hybridization, and microarray washing were performed according to manufacturer’s instructions (Agilent Technologies).
|
| Scan protocol |
Scanned using a GenePix 4000A Scanner (Axon Instruments-Molecular Devices).
|
| Description |
Subject 4 after LCD
|
| Data processing |
Microarray normalization was carried out by subtracting the median intensity background signal prior to intra-slide Loess normalization of log-transformed data (Goulphar Version 1.1.3 package). All data was then uploaded into the JMP Genomics Version 4.1 platform and further normalized using a quantile inter-slide intensity method.
|
| |
|
| Submission date |
Sep 29, 2010 |
| Last update date |
May 04, 2012 |
| Contact name |
David M Mutch |
| E-mail(s) |
[email protected]
|
| Phone |
+1-519-824-4120
|
| Organization name |
University of Guelph
|
| Department |
Human Health & Nutritional Sciences
|
| Lab |
ANNU 308
|
| Street address |
50 Stone Road East
|
| City |
Guelph |
| State/province |
Ontario |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE24432 |
Subcutaneous adipose tissue: comparison of weight maintainence and weight regain following an 8-week low calorie diet |
|
