|
| Status |
Public on May 01, 2011 |
| Title |
heart_wild_type_sham_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Whole Mouse Reference RNA (WMRR)
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed 129Sv and C57BL/6
developmental stage: neonatal
tissue: whole body
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol (Invitrogen) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
500 ng of WMRR (PMID 15470901) or heart RNA labeled with Cy3 or Cy5 (Perkin Elmer), respectively, and amplified using the Low Input RNA Amplification Kit (Agilent) as described by the manufacturer.
|
| |
|
| Channel 2 |
| Source name |
heart, 1 week post sham surgery
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129Sv
genotype: wild-type
tissue: heart ventricle
surgery: sham
time post-surgery: 1 week
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol (Invitrogen) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
500 ng of WMRR (PMID 15470901) or heart RNA labeled with Cy3 or Cy5 (Perkin Elmer), respectively, and amplified using the Low Input RNA Amplification Kit (Agilent) as described by the manufacturer.
|
| |
|
| |
| Hybridization protocol |
Cy5-labeled cRNA was co-hybridized to Agilent-014868 Whole Mouse Genome Microarrays with equimolar amounts of Cy3-labeled WMRR-derived cRNA. Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
| Scan protocol |
Scanned on an Axon Instruments GenePix 4000b scanner and processed using Agilent FeatureExtractor 9.1.3.1.
|
| Description |
CHIPtab02
Biological replicate 2 of 4.
|
| Data processing |
Data files uploaded into GeneSpring 11.0.2 using a median value baseline transformation. Entities where <50 percent of samples in any 1 out of 4 conditions have flags of Present or Marginal were filtered and removed.
|
| |
|
| Submission date |
Sep 17, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Jonathan C Schisler |
| E-mail(s) |
[email protected]
|
| Phone |
919-843-8708
|
| Organization name |
The University of North Carolina at Chapel Hill
|
| Department |
McAllister Heart Institute
|
| Lab |
Schisler Lab
|
| Street address |
MBRB, Rm 2340C
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7126 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE24209 |
Effect of pressure overload on cardiac gene expression in wild-type and CHIP(-/-) mice |
|
