|
| Status |
Public on Nov 03, 2021 |
| Title |
SMC-TNFa |
| Sample type |
RNA |
| |
|
| Source name |
aorta smooth muscle cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Aorta smooth muscle cells
treatment: TNFa
|
| Treatment protocol |
Cells were starved for 24 hours before adding cytokines (PDGFBB 40ug/ml; TGFb 20ug/ml; IL1b 10 ug/ml; TNFa 20ug/ml), stimulated for 24 hours before harvest.
|
| Growth protocol |
Human aortic smooth muscle cells (Lonza, cat #: CC-2571) were cultured in smooth muscle cell growth medium (SmGM, Lonza #CC-3182) with supplements.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the TRIzol reagent (15596026) following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA is synthesized from total RNA. An in vitro transcription (IVT) reaction is then done to produce biotin-labeled cRNA from the cDNA.
|
| |
|
| Hybridization protocol |
The fragmented target and probe array controls were hybridized for 16 hours.
|
| Scan protocol |
Once the probe array has been hybridized, washed, and stained, it is scanned. Each complete probe array image is stored in a separate data file identified by the experiment name.
|
| Description |
Gene expression data from human aorta smooth muscle cells treated with TNFa.
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TCA4.0) using default analysis setting and normalization method.
|
| |
|
| Submission date |
Oct 12, 2021 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Xiujie Xie |
| E-mail(s) |
[email protected]
|
| Phone |
6142645638
|
| Organization name |
university of virginia
|
| Street address |
409 Lane Rd MR4
|
| City |
charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22903 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE185784 |
miR548ai antagonism attenuates exosome-induced endothelial cell dysfunction |
|
