|
| Status |
Public on Sep 27, 2019 |
| Title |
MEFs |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
cell type: cultured embryonic skin fibroblasts
developmental stage: n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using NucleoSpin RNA XS (Takara, Japan) and quantified using a NanoDrop-1000 spectrophotometer. The quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 100 ug RNA using the Low Input Quick Amp WT Labeling Kit, One-Color*3 (Agilent) according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Total RNA was amplified using the Low Input Quick Amp WT Labeling Kit, One-Color*3 (Agilent), according to the manufacturer’s instructions (Agilent Technologies, Santa Clara, USA). 100 ng of total RNA was reverse-transcribed into double-stranded cDNA, which was translated into cRNA in the presence of Cy3-dCTP and amplified by T7 RNA polymerase.
|
| Scan protocol |
Scaned using microarray scanner G2690D
|
| Description |
cultured embryonic skin fibroblasts
|
| Data processing |
All scanned images were analyzed using Agilent feature extraction software (v11.5.1.1). Raw data were imported with GeneSpring GX software (v14.9, Agilent Technologies, CA, USA) with adapted by 75 percentile shift normalization
|
| |
|
| Submission date |
Mar 26, 2019 |
| Last update date |
Sep 27, 2019 |
| Contact name |
Yasuhiro Nakano |
| E-mail(s) |
[email protected]
|
| Phone |
813-5803-4658
|
| Organization name |
Tokyo Medical and Dental University (TMDU)
|
| Street address |
1-5-45 Yushima
|
| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE128895 |
A novel deactivation factor of fibrogenic hepatic stellate cells induces regression of liver fibrosis in mice |
|
