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| Status |
Public on Sep 27, 2019 |
| Title |
A novel deactivation factor of fibrogenic hepatic stellate cells induces regression of liver fibrosis in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
It has been reported that hepatic stellate cells (HSCs) differentiate from mesodermal-derived submesothelial cells during embryonic development, and that these cells express a common surface marker p75 neurotrophin receptor (p75NTR). We sorted p75NTR-expressing cells in embryonic liver at each developmental stage, and transcription profiles were analyzed using the DNA microarray.
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| Overall design |
Liver tissues obtained from E11.5, E13.5, E14.5, and E17.5 fetuses or newborn mice (P1) were excised under a microscope and dissociated in 0.05% collagenase solution. The CD45(−)Ter119(−)p75NTR(+) cell fraction was obtained by FACS. Total RNA was prepared from isolated these cells and transcription profiles were analyzed using the DNA microarray. Hierarchical clustering of normalized signal intensities was performed using Euclidean distances and centroid linkage. Raw intensity values were normalized using the 75th percentile and transformed to log2 scale.
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| Contributor(s) |
Nakano Y, Kamiya A, Inagaki Y |
| Citation(s) |
31549421 |
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| Submission date |
Mar 26, 2019 |
| Last update date |
Sep 29, 2019 |
| Contact name |
Yasuhiro Nakano |
| E-mail(s) |
[email protected]
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| Phone |
813-5803-4658
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| Organization name |
Tokyo Medical and Dental University (TMDU)
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| Street address |
1-5-45 Yushima
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| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA529232 |
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