|
| Status |
Public on Feb 13, 2019 |
| Title |
Primary hippocampal cultures_GYKI-52466 + MK-801 9h_R2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary cultures of hippocampal neurons, GYKI-52466 + MK-801, 9h
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: Hippocampus
tissue type: Primary cultures of hippocampal neurons
days in vitro (div): 14-15
treatment: 50 μM GYKI-52466 + 10 μM MK-801 for 9h
|
| Treatment protocol |
Hippocampal cultures were treated with 50 μM GYKI-52466 and 10 μM MK-801 for 9 or 26 hours or kept in control conditions.
|
| Growth protocol |
Primary cultures of hippocampal neurons were prepared from embryonic day (E) 18 Wistar rats. The cultures were mantained in Neurobasal medium [supplemented with SM1 neuronal supplement (1:50), glutamine (0.5mM), gentamycin (0.12 mg/ml) and glutamate (25 μM, first week in culture)] and kept in a humidified incubator at 37º with 5% CO2/95% air, for 14-15 day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from three independent rat hippocampal neuronal cultures subjected either to chronic synaptic activity blockade for 9h or 26h, or under control conditions was extracted using TRIzol (according to manufecturer's instructions).
|
| Label |
Cy3
|
| Label protocol |
Equal amounts of RNA extract (200 ng) from each replicate were amplified and Cy-3-labeled using the Low Input Quick Amp Labeling kit (Agilent Technologies), according to manufecturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridizations were carried out following Agilent Technologies instructions for One-Color Microarray-Based Gene Expression Analysis (Agilent Technologies, Santa Clara, CA, USA), using whole-genome Rat GE 4x44K v3 Microarrays.
|
| Scan protocol |
Images were obtained using Agilent G2565AA microarray scanner and fluorescence quantization was performed using Agilent Feature Extraction 10.5.1.1 software and GE1_105_Dec08 protocol.
|
| Description |
Gene expression of hippocampal cultures treated with AMPARs and synaptic NMDARs antagonists _ 9h
|
| Data processing |
The signal intensity was aligned and normalized between microarrays by centering the median of the signal distribution using BRB-ArrayTools v3.8.1.
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| |
|
| Submission date |
Mar 03, 2018 |
| Last update date |
Feb 14, 2019 |
| Contact name |
Joana Filipa Fernandes |
| E-mail(s) |
[email protected]
|
| Organization name |
Center for Neuroscience and Cell Biology
|
| Department |
Life Sciences
|
| Lab |
Neurobiology and Disease
|
| Street address |
Rua Larga, Faculdade de Medicina 2º andar, lab 206A Coimbra
|
| City |
Coimbra |
| State/province |
-- Please Select -- |
| ZIP/Postal code |
3004-504 |
| Country |
Portugal |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE111384 |
Synaptic scaling regulates the transcriptome in hippocampal neurons |
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