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| Status |
Public on Feb 13, 2019 |
| Title |
Synaptic scaling regulates the transcriptome in hippocampal neurons |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To better comprehend how synaptic scaling affects the neuronal transcriptome, we used the whole genome microarray expression profiling in hippocampal cultures treated with AMPARs and synaptic NMDARs antagonists for 9h and 26h, or under control conditions. Gene ontology enrichment analysis showed altered transcripts associated with synaptic signalling and synaptic plasticity classes, including transcripts of several proteins already associated with synaptic scaling mechanisms.
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| Overall design |
The effects of chronic synaptic activity blockade in gene expression were analyzed in primary cultures of rat hippocampal neurons treated for 9h or 26h with AMPARs antagonist GYKI-52466 and synaptic NMDARs antagonist MK-801. Three independent experiments were performed at each time (9 or 26 hours) as independent biological replicates.
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| Contributor(s) |
Silva MM, Rodrigues B, Fernandes J, Santos SD, Carreto L, Santos MA, Pinheiro P, Carvalho A |
| Citation(s) |
30808806 |
| |
| Submission date |
Mar 03, 2018 |
| Last update date |
May 15, 2019 |
| Contact name |
Joana Filipa Fernandes |
| E-mail(s) |
[email protected]
|
| Organization name |
Center for Neuroscience and Cell Biology
|
| Department |
Life Sciences
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| Lab |
Neurobiology and Disease
|
| Street address |
Rua Larga, Faculdade de Medicina 2ยบ andar, lab 206A Coimbra
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| City |
Coimbra |
| State/province |
-- Please Select -- |
| ZIP/Postal code |
3004-504 |
| Country |
Portugal |
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| Platforms (1) |
| GPL14746 |
Agilent-028282 Whole Rat Genome Microarray 4x44K v3 (Probe Name version) |
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| Samples (12)
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| GSM3029831 |
Primary hippocampal cultures_Ctrl 9h_R1 |
| GSM3029832 |
Primary hippocampal cultures_Ctrl 9h_R2 |
| GSM3029833 |
Primary hippocampal cultures_Ctrl 9h_R3 |
| GSM3029834 |
Primary hippocampal cultures_GYKI-52466 + MK-801 9h_R1 |
| GSM3029835 |
Primary hippocampal cultures_GYKI-52466 + MK-801 9h_R2 |
| GSM3029836 |
Primary hippocampal cultures_GYKI-52466 + MK-801 9h_R3 |
| GSM3029837 |
Primary hippocampal cultures_Ctrl 26h_R1 |
| GSM3029838 |
Primary hippocampal cultures_Ctrl 26h_R2 |
| GSM3029839 |
Primary hippocampal cultures_Ctrl 26h_R3 |
| GSM3029840 |
Primary hippocampal cultures_GYKI-52466 + MK-801 26h_R1 |
| GSM3029841 |
Primary hippocampal cultures_GYKI-52466 + MK-801 26h_R2 |
| GSM3029842 |
Primary hippocampal cultures_GYKI-52466 + MK-801 26h_R3 |
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| Relations |
| BioProject |
PRJNA450745 |
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