|
| Status |
Public on Nov 30, 2018 |
| Title |
HSD1+BVT |
| Sample type |
RNA |
| |
|
| Source name |
cardiomyocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
developmental stage: neonatal
cell type: ventricular cardiomyocytes
treatment: co-treated with overexpression 11β-HSD1 vector and 11β-HSD1 inhibitor BVT.2733
|
| Treatment protocol |
Neonatal rat ventricular cardiomyocytes were transfected either with the expression vector for 11β-HSD1 or co-treated with overexpression 11β-HSD1 vector and 11β-HSD1 inhibitor BVT.2733, or transfected with corresponding empty vector.
|
| Growth protocol |
The neonatal rat ventricular cardiomyocytes were cultured at 37 °C in a 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A pool containing equivalent amounts of mixed cardiomyocytes for each group was prepared and purified using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
A total of 1 µg RNA from each group was linearly amplified and labeled with Cy3-UTP.
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized to Agilent Whole Rat Genome Microarray (Agilent, 4×44 K) using an Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242) according to the manufacturer’s protocol.
|
| Scan protocol |
The processed slides were scanned with an Agilent DNA microarray scanner (Agilent p/n G2565BA).
|
| Description |
neonatal rat ventricular cardiomyocytes co-treated with overexpression 11β-HSD1 vector and 11β-HSD1 inhibitor BVT.2733
|
| Data processing |
The raw gene expression data were extracted from Agilent Feature Extraction Software (version 11.0.1.1) and imported into Agilent GeneSpring GX software (version12.1) for further analysis.
|
| |
|
| Submission date |
Dec 01, 2017 |
| Last update date |
Nov 30, 2018 |
| Contact name |
MIN HUANG |
| E-mail(s) |
[email protected]
|
| Phone |
8615851808138
|
| Organization name |
The First Hospital Affiliated to Nanjing Medical University
|
| Department |
Geriatrics
|
| Street address |
300 GUANGZHOU ROAD
|
| City |
NANJING |
| State/province |
JIANGSU |
| ZIP/Postal code |
210029 |
| Country |
China |
| |
|
| Platform ID |
GPL14746 |
| Series (1) |
| GSE107587 |
RNA expression profiling in cardiomyocytes |
|
