|
| Status |
Public on Jan 12, 2018 |
| Title |
SUM44_LTED_repl2 |
| Sample type |
SRA |
| |
|
| Source name |
breast cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer cell line
biomaterial provider: ATCC
cell line: SUM44
estrogen: No, LTED (long term estrogen deprived)
esr1 mutation: Y537S
|
| Treatment protocol |
LTED cells modeling resistance to an AI were derived from long-term culture in the presence of RPMI1640 medium containing 10% dextran charcoal stripped serum (DCC)
|
| Growth protocol |
cultured in phenol red-free RPMI1640 medium supplemented with 10% FBS and 1 nmol/L estradiol (E2)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy kit (Qiagen)
Libraries were created after Ribo-zero rRNA removal kit (Illumina) using NEBNext Ultra Directional RNA (NEB) kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
SUM44 LTED replicate 2
|
| Data processing |
Tophat (v2.1) and Cuffdiff (v2.2.1) using default parameters
Genome_build: Human Reference Genome (assembly hg19)
Supplementary_files_format_and_content: Cuffdiff output files
|
| |
|
| Submission date |
Jun 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Eugene Schuster |
| E-mail(s) |
[email protected]
|
| Phone |
+442088082619
|
| Organization name |
Institute of Cancer Research
|
| Department |
Breast Cancer Research
|
| Lab |
Endocrinology
|
| Street address |
123 Old Brompton Road
|
| City |
London |
| ZIP/Postal code |
SW7 3RP |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE100075 |
Discovery of naturally occurring ESR1 mutations during acquisition of resistance to endocrine therapy in widely used estrogen receptor positive breast cancer cell lines [RNA-Seq] |
| GSE100076 |
Discovery of naturally occurring ESR1 mutations during acquisition of resistance to endocrine therapy in widely used estrogen receptor positive breast cancer cell lines |
|
| Relations |
| BioSample |
SAMN07246994 |
| SRA |
SRX2922250 |
