|
| Status |
Public on Sep 26, 2017 |
| Title |
FRT82B_d5_S3_mRNA |
| Sample type |
SRA |
| |
|
| Source name |
wing discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
treatment: hand dissections
tissue: wing discs
genotype/variation: y w ubxflp/ y w; FRT82B M(3) ubiGFP/ FRT82B
age: 3rd instar-day5
|
| Growth protocol |
Normal fly food, 25 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Wing discs were collected from 12-35 larvae under sterile conditions and immediately lysed. RNA extraction was done using Ambion RNAqueous Micro kit.
Illumina TruSeq mRNA Library Prep reagents used according to the protocol recommended by the manufacturer.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
mRNA
Processed data file: RNA_seq_rawcounts_18samples.tsv
|
| Data processing |
Cleaning (fastq-mcf).
Mapping to the dm6 genome using bowtie2 tophat2.
Obtaining raw counts per gene using htseq-count.
Library size normalisation and differential expression using R DESeq2.
Genome_build: Drosophila melanogaster FlyBase version 6.03
Supplementary_files_format_and_content: RNA_seq_rawcounts_18samples.tsv: Tab-separated text file includes raw counts matrix dmel 6.03.
|
| |
|
| Submission date |
Mar 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jelle Jacobs |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of molecular Pathology
|
| Department |
VBC
|
| Lab |
Stark lab
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
| GSE96868 |
Hippo reprograms the transcriptional response to Ras signalling |
|
| Relations |
| Reanalysis of |
GSM1832938 |
| Reanalyzed by |
GSM3283109 |
| BioSample |
SAMN06624344 |
| SRA |
SRX2659817 |
