|
| Status |
Public on Aug 01, 2017 |
| Title |
Western-Diet-Females-2 |
| Sample type |
RNA |
| |
|
| Source name |
small intestine
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J Min/+
gender: Female
tissue: small intestine
diet: Diet modified from AIN-93G, with high fat and low fiber, vitamin D, calcium and folate. (66.4% of total fat from milk, and 33.6% from rapeseed and sunflower oil)
|
| Treatment protocol |
an 18 months feeding experiment
|
| Growth protocol |
standard conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted from small intestine using the RNeasy Mini kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
The agilent chip labeling, hybridization and scanning were performed at the Biomedicum Functional Genomics Unit, University of Helsinki using standard agilent procedures
|
| |
|
| Hybridization protocol |
The agilent chip labeling, hybridization and scanning were performed at the Biomedicum Functional Genomics Unit, University of Helsinki using standard agilent procedures
|
| Scan protocol |
The agilent chip labeling, hybridization and scanning were performed at the Biomedicum Functional Genomics Unit, University of Helsinki using standard agilent procedures
|
| Data processing |
The data was normalized using mean normalization, and genes with detectable expression in four chips were used in further analyses
|
| |
|
| Submission date |
Aug 17, 2016 |
| Last update date |
Aug 01, 2017 |
| Contact name |
Markus Storvik |
| Organization name |
University of Eastern Finland
|
| Department |
Pharmacology
|
| Street address |
University of Eastern Finland
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE85729 |
Increased tumourigenesis in ApcMin/+ mice fed with a Western-type diet associates with changes in PDK4, Erk/Akt and mTor pathways, intestinal immunity and the expression of energy metabolism-related genes |
|
