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| Status |
Public on May 12, 2016 |
| Title |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines [HTS] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cancer stem cells (CSC) dictate tumor cell heterogeneity in diverse cancer types and arise, in part, from microRNA (miRNA)-dependent alteration of gene expression. The let-7 miRNA family induces differentiation by silencing genes that maintain stemness and is repressed by the RNA-binding proteins LIN28A/B, which preserve stemness in normal embryonic and malignant cells. Here, we observed that LIN28A/B is undetectable in glioma stem cells (GSC) whereas let-7 and, paradoxically, their target genes are highly expressed. Using photoactivatable-ribonucloside-enchanced crosslinking and immunoprecipitation (PAR-CLIP), we show that insulin-like growth factor-2 mRNA-binding protein 2 (IMP2) protects let-7 target genes from silencing and provides a mechanistically distinct alternative to LIN28A/B toward both GSC and neural stem cell specification. Our observations define the RNA-binding repertoire of IMP2 and identify a mechanism by which it supports GSC maintenance.
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| Overall design |
We characterized RNA binding sites of IMP2 in MGG8 Glioma cancer stem cells and differentiated cells. To profile changes upon IMP2 modulation we performed RNA-Seq in MGG4 and 8 cells in both states upon IMP2 knockdown and overexpression. Furthermore we investigated changes during differentiation from cancer stem cells to adherent cells in 6 and 7 RNA-Seq datasets in MGG4 and 8 cells.
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| Contributor(s) |
Degrauwe N, Schlumpf TB, Paro R, Stamenkovic I |
| Citation(s) |
27184842 |
| |
| Submission date |
Oct 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Tommy Beat Schlumpf |
| Organization name |
ETH Zürich
|
| Department |
D-BSSE
|
| Lab |
Paro
|
| Street address |
Mattenstrasse 26
|
| City |
Basel |
| State/province |
Basel-Stadt |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (25)
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| This SubSeries is part of SuperSeries: |
| GSE73847 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines |
|
| Relations |
| BioProject |
PRJNA298216 |
| SRA |
SRP064610 |
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