|
| Status |
Public on May 12, 2016 |
| Title |
RNA-Seq_mm_ED15_KO [Sample 21] |
| Sample type |
SRA |
| |
|
| Source name |
ED15 IMP2fl/fl nestin cre brain
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6
age: Embryonic day 15
genotype/variation: IMP2 floxed/floxed but crossed to Nestin Cre (i.e part of IMP2 is removed from the genome in tissues with Nestin expression)
tissue: brain
|
| Growth protocol |
NeuroCult NSC Basal Medium (ref. 05700, Stemcell Technologies) supplemented with 10% Neurocult NSC Proliferation Supplement (ref. 05701, Stemcell Technologies) and 20ng/mL EGF
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA extraction kit Exiqon, on colum DNASE treatment (Qiagen)
Illumina polyA stranded
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
processed data: RNA_brain_IMP2_KO.txt
|
| Data processing |
Genomic alignment with TopHat 2.0.12, --b2-very-sensitive --library-type fr-firststrand --b2-N 1
Differential expression calling with edgeR 3.10.2 and R 3.2.0
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited textfile containing the raw readcounts, normalized cpm and gene fold-changes
|
| |
|
| Submission date |
Oct 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Tommy Beat Schlumpf |
| Organization name |
ETH Zürich
|
| Department |
D-BSSE
|
| Lab |
Paro
|
| Street address |
Mattenstrasse 26
|
| City |
Basel |
| State/province |
Basel-Stadt |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE73845 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines [HTS] |
| GSE73847 |
Functional characterization of RNA-binding protein IMP2 in primary Glioma cell lines |
|
| Relations |
| BioSample |
SAMN04156668 |
| SRA |
SRX1317336 |
