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| Status |
Public on May 27, 2015 |
| Title |
Drosophila Imp iCLIP identifies an RNA assemblage co-ordinating F-actin formation |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we employed a combination of RIP-seq and short- and long-wave iCLIP technologies to identify transcripts associated with cytoplasmic RNPs containing the RNA-binding protein Drosophila Imp. We also made a Imp knockdown vs luciferase control experiment.
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| Overall design |
Two biological iCLIP-seq, as well as two mRNAseq made on cells harvested on the same day. Two biological PAR-iCLIP-seq. Two biological RIP-seq, as well as two mRNAseq made on cells harvested on the same day. Three biological mRNAseq of imp dsRNA treated cells as well as three control mRNAseq of cells treated with Luciferase dsRNA.
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| Contributor(s) |
Hansen HT, Rasmussen SH, Adolph SA, Plass M, Krogh A, Sanford J, Nielsen FC, Christiansen J |
| Citation(s) |
26054396 |
| |
| Submission date |
Nov 05, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Mireya Plass |
| E-mail(s) |
[email protected]
|
| Organization name |
MDC-Berlin
|
| Street address |
Robert-Rössle Str. 10
|
| City |
Copenhagen |
| ZIP/Postal code |
13092 |
| Country |
Germany |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA266435 |
| SRA |
SRP049555 |
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