|
| Status |
Public on Jun 30, 2012 |
| Title |
Major concepts of piRNA biogenesis revealed by the analysis of Shutdown, a co-chaperone with essential roles in the biogenesis of all Drosophila piRNA populations |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In animal gonads, 23-30nt long PIWI interacting RNAs (piRNAs) guarantee genome integrity by guiding the sequence specific silencing of selfish genetic elements such as transposons. Two major branches of piRNA biogenesis, namely primary processing and ping-pong amplification, feed into the PIWI clade of Argonaute proteins. Despite our conceptual understanding of piRNA biogenesis, major gaps exist in the mechanistic understanding of the underlying molecular processes as well as in the knowledge of the involved players. Here, we demonstrate an essential role for the female sterility gene shutdown in the piRNA pathway. Shutdown, an evolutionarily conserved co-chaperone of the immunophilin class is the first piRNA biogenesis factor that is essential for all primary and secondary piRNA populations in Drosophila. Based on these findings, we define distinct groups of piRNA biogenesis factors and reveal the core concept of how PIWI family proteins are hard-wired into piRNA biogenesis processes.
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| |
|
| Overall design |
small-RNA libraries from 2 control samples and 7 knock-down samples of D. mel. ovaries and 2 small-RNA profiles from Piwi IP and Aub IP from OSCs.
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| |
|
| Contributor(s) |
Brennecke J, Olivieri D |
| Citation(s) |
22902557 |
| |
| Submission date |
Jun 14, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Julius Brennecke |
| E-mail(s) |
[email protected]
|
| Organization name |
IMBA
|
| Street address |
Dr. Bohrgasse 3
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (13)
|
| GSM948741 |
small RNAs (18-29nt) from MTD>w- |
| GSM948742 |
small RNAs (18-29nt) from MTD>GFP shRNA |
| GSM948743 |
small RNAs (18-29nt) from MTD>armi shRNA |
| GSM948744 |
small RNAs (18-29nt) from MTD>zuc shRNA |
| GSM948745 |
small RNAs (18-29nt) from MTD>piwi shRNA |
| GSM948746 |
small RNAs (18-29nt) from MTD>vas shRNA |
| GSM948747 |
small RNAs (18-29nt) from MTD>aub shRNA |
| GSM948748 |
small RNAs (18-29nt) from MTD>vret shRNA |
| GSM948749 |
small RNAs (18-29nt) from UAS-Dcr-2;NGT;nanosGAL4>shu RNAi |
| GSM948750 |
small RNAs from FLAG_HA_Piwi Immunoprecipitation |
| GSM948751 |
small RNAs from FLAG_HA_Aub Immunoprecipitation |
| GSM948752 |
small RNAs (18-29nt) from shu[1]/CyO hets and shu[Def]/CyO hets. mixed |
| GSM948753 |
small RNAs (18-29nt) from shu[1]/shu[Def] mutants |
|
| Relations |
| BioProject |
PRJNA168504 |
| SRA |
SRP013751 |
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