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| Status |
Public on Nov 06, 2011 |
| Title |
Expression data from 6 day-old Atxn1L-/- and Atxn1-/- lung tissues |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Although expansion of a polyglutamine tract in ATAXIN1 (ATXN1) causes Spinocerebellar ataxia type 1, the functions of wild-type ATXN1 and ATAXIN1-Like (ATXN1L) remain poorly understood. To gain insight into the function of these proteins, we generated and characterized Atxn1L-/- and Atxn1-/- ; Atxn1L-/- double mutant animals. We found that Atxn1L -/- mice have several developmental problems including hydrocephalus, omphalocoele and lung alveolarization defects. These phenotypes are more penetrant and severe in Atxn1-/- ; Atxn1L-/- mice, suggesting that Atxn1 and Atxn1L are functionally redundant.
To unravel the molecular mechanism underlying the alveolarization defect in Atxn1-/- mice, we carried out microarray analyses using total lung RNA from WT, Atxn1-/- and Atxn1L-/- mice
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| Overall design |
Lung tissues were dissected from 6 day old mice from either Atxn1 heterozyous intercrosses or Atxn1L heterozygous intercrosses. We collected lung tissue samples from 4 pairs of WT and Atxn1-/- amd 3 pairs of WT and Atxn1-/- mice. Total RNA extracted from the samples was subjected to microarray analysis using Affymetrix mouse gene ST 1.0 array.
GSM731591-GSM731598 (Atxn1L KO and controls) and GSM731599-GSM731604 (Atxn1 KO and controls) were RMA-processed separately.
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| Contributor(s) |
Lee Y, Kang H, Bowman AB, Zoghbi HY |
| Citation(s) |
22014525 |
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| Submission date |
May 26, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Hyojin Kang |
| E-mail(s) |
[email protected]
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| Phone |
832-824-8907
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| Fax |
713-798-8728
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| Organization name |
Baylor College of Medicine
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| Department |
Molecular and Human Genetics
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| Lab |
Huda Y. Zoghbi Lab
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| Street address |
One Baylor Plaza
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| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL11078 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENTREZG_13] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA141315 |
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