|
| Status |
Public on Nov 06, 2011 |
| Title |
Atxn1 KO lung rep1 |
| Sample type |
RNA |
| |
|
| Source name |
lung, Atxn1 knock-out
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
genotype: Atxn1 -/-
age: P6
strain: C57BL/6J
|
| Treatment protocol |
Lung tissues were dissected from P6 mice and quickly frozen with liquid nitrogen.
|
| Growth protocol |
Mice were maintained under the regulation of the Institutional Animal Care and Use Committee (IACUC) at Baylor College of Medicine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA was subjected to the Affymetrix Gene ST labelling (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from mouse lung tissues at P6
|
| Data processing |
The data were analyzed using Limma package in the Bioconductor R. The signal intensities were obtained and corrected for background by using Robust Multi-array Analysis (RMA) algorithm.
|
| |
|
| Submission date |
May 26, 2011 |
| Last update date |
Nov 06, 2011 |
| Contact name |
Hyojin Kang |
| E-mail(s) |
[email protected]
|
| Phone |
832-824-8907
|
| Fax |
713-798-8728
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Lab |
Huda Y. Zoghbi Lab
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL11078 |
| Series (1) |
| GSE29551 |
Expression data from 6 day-old Atxn1L-/- and Atxn1-/- lung tissues |
|
