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Sample GSM731604 Query DataSets for GSM731604
Status Public on Nov 06, 2011
Title WT ctrl for Atxn1 KO rep3
Sample type RNA
 
Source name lung, WT
Organism Mus musculus
Characteristics tissue: lung
genotype: WT
age: P6
strain: C57BL/6J
Treatment protocol Lung tissues were dissected from P6 mice and quickly frozen with liquid nitrogen.
Growth protocol Mice were maintained under the regulation of the Institutional Animal Care and Use Committee (IACUC) at Baylor College of Medicine
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 100 ng of total RNA was subjected to the Affymetrix Gene ST labelling (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Affymetrix standard protocol
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data from mouse lung tissues at P6
Data processing The data were analyzed using Limma package in the Bioconductor R. The signal intensities were obtained and corrected for background by using Robust Multi-array Analysis (RMA) algorithm.
 
Submission date May 26, 2011
Last update date Nov 06, 2011
Contact name Hyojin Kang
E-mail(s) [email protected]
Phone 832-824-8907
Fax 713-798-8728
Organization name Baylor College of Medicine
Department Molecular and Human Genetics
Lab Huda Y. Zoghbi Lab
Street address One Baylor Plaza
City Houston
ZIP/Postal code 77030
Country USA
 
Platform ID GPL11078
Series (1)
GSE29551 Expression data from 6 day-old Atxn1L-/- and Atxn1-/- lung tissues

Data table header descriptions
ID_REF
VALUE log2 RMA-signal

Data table
ID_REF VALUE
100009600_at 7.747557741
100009609_at 5.35681793
100012_at 6.275570501
100017_at 10.73230505
100019_at 9.545531128
100033459_at 7.799849365
100034251_at 12.47366348
100036521_at 9.287620202
100037258_at 11.6231362
100037278_at 9.117963304
100038380_at 8.877941657
100038429_at 8.7489261
100038486_at 7.435810327
100038570_at 8.830319428
100038598_at 8.316750178
100038600_at 9.212119171
100038618_at 8.583447797
100038635_at 9.683393575
100038680_at 7.790114798
100038683_at 8.386701337

Total number of rows: 21212

Table truncated, full table size 442 Kbytes.




Supplementary file Size Download File type/resource
GSM731604_wt_rep3_27813.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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