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| Status |
Public on Jun 30, 2005 |
| Title |
Auxin induction in wild type and arf6 arf8 flowers |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim of this study was to examine the roles of Auxin Response Factors (ARFs) in flower gene expression. Flowers from arf6 arf8 plants undergo a developmental arrest at approximately stage 12, just prior to flower opening. Wild-type, ARF6/arf6 arf8/arf8, and arf6 arf8 plants were treated with 10 uM indole-3-acetic acid for thirty minutes to identify genes that respond rapidly to auxin in an ARF6/ARF8-dependent manner.
Keywords: auxin response; comparison of wild type and arf6 arf8 mutants
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| Overall design |
Wild-type, ARF6/arf6 arf8/arf8, and arf6 arf8 plants were sprayed with 10 mM indole-3-acetic acid in 1% methanol, 0.05% Tween-20. Thirty minutes after treatment, flowers (stage 1-14) were collected, frozen in liquid nitrogen, and used for RNA extraction.
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| Contributor(s) |
Nagpal P, Ellis CM, Reed JW |
| Citation(s) |
16107481 |
| |
| Submission date |
Jun 28, 2005 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Christine Ellis |
| Organization name |
University of North Carolina at Chapel Hill
|
| Street address |
121 Coker Hall
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-3280 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (7)
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| GSM62687 |
Columbia flowers_stage 1-14_untreated |
| GSM62688 |
Columbia flowers_stage 1-14_IAA |
| GSM62689 |
Columbia flowers_stage 1-14_mock |
| GSM62690 |
arf6/arf6 ARF8/arf8 flowers_stage 1-14_untreated |
| GSM62691 |
arf6/arf6 ARF8/arf8 flowers_stage 1-14_IAA |
| GSM62692 |
arf6 arf8 flowers_stage 1-14_untreated |
| GSM62693 |
arf6 arf8 flowers_stage 1-14_IAA |
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| Relations |
| BioProject |
PRJNA92567 |
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