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| Status |
Public on Dec 18, 2020 |
| Title |
Nascent chain interactions of proximal ribosomes drive homo-oligomer assembly |
| Organisms |
Escherichia coli; Homo sapiens |
| Experiment type |
Other
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| Summary |
Accurate assembly of newly- synthesized proteins into functional oligomers is crucial for cell activity. In this study, we investigated whether direct interaction of two nascent proteins, emerging from nearby ribosomes (co-co assembly), constitutes a general mechanism for oligomer formation. We used a proteome-wide screen to detect nascent chain-connected ribosome pairs and identified hundreds of homomer subunits that co-co assemble in human cells. Interactions are mediated by five major domain classes, among which N-terminal coiled coils are the most prevalent. We were able to reconstitute co-co assembly of nuclear lamin in Escherichia coli, demonstrating that dimer formation is independent of dedicated assembly machineries. Co-co assembly may thus represent an efficient way to limit protein aggregation risks posed by diffusion-driven assembly routes and ensure isoform-specific homomer formation.
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| Overall design |
Screen of nascent chain dimerization in two human cell lines (HEK293-T and U2OS, 2 replicates each). Total translatomes from HEK293-T cells are also provided in 2 replicates. Similar screen performed with treatment of HEK293-T cell lysates with puromycin (2 replicates) or a titration of Proteinase K (4 conditions, 1 replicate). Similar screen performed in E.coli over-expressing a human gene candidate (5 genes tested, 4 of which are performed in 2 replicates).
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| Contributor(s) |
Bertolini M, Fenzl K, Wruck F, Kats I, Tippmann F, Schmitt J, Auburger J, Tans S, Bukau B, Kramer G |
| Citation(s) |
33384371 |
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| Submission date |
Jun 07, 2020 |
| Last update date |
Mar 19, 2021 |
| Contact name |
Kai Fenzl |
| E-mail(s) |
[email protected]
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| Organization name |
ZMBH
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| Lab |
Prof. Bernd Bukau
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| Street address |
INF 282
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| City |
Heidelberg |
| ZIP/Postal code |
69115 |
| Country |
Germany |
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| Platforms (3) |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA637897 |
| SRA |
SRP266332 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151959_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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