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| Status |
Public on Dec 31, 2019 |
| Title |
Clariom D expression in breast cancer samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We analysed expression using Clariom D array in 42 breast cancer FFPE samples
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| Overall design |
The RNA for the Clariom™ D study was extracted from tissue fixed in formaldehyde and embedded in paraffin, preserved at room temperature. Four sections of 20 μm from breast cancer tumour paraffin embedded were used. The extraction of the RNA material was carried out using the commercial kit RecoverAll ™ Total Nucleic Acid Isolation Kit from ambion® (Applied Biosystems ™ by Life Technologies ™, Carlsbad, California, USA). RNA was quantified by Qubit® 3.0 (InvitrogenTM by Termo Fisher Scientific, Waltham, MA, USA) y Qubit® RNA HS Assay Kit (Molecular Probes® by Life Technologies Carlsbad, California, USA). Before hybridization RNA quality was assessed using Real Time PCR (qRT-PCR).
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| Contributor(s) |
Oltra SS, Peña-Chilet M, Vidal-Tomas V, Flower K, Martinez MT, Alonso E, Burgues O, Lluch A, Flanagan JM, Ribas G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 25, 2019 |
| Last update date |
Jan 01, 2020 |
| Contact name |
Sara OLTRA SANCHIS |
| E-mail(s) |
[email protected]
|
| Phone |
961973540
|
| Organization name |
INCLIVA
|
| Department |
Medical Oncology
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| Lab |
Cancer Functional Genomics
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| Street address |
Avda Menéndez Pelayo, 4 accesorio
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA524163 |
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