|
| Status |
Public on Jul 01, 2018 |
| Title |
Increasing miRNA sequencing accuracy using an RNA circularization approach |
| Organisms |
Homo sapiens; synthetic construct |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Accurate quantification of all microRNAs (miRNA) is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We have developed a new method of preparation of small RNA sequencing libraries (RealSeq®-AC) that ligates miRNAs with a single combo adapter and then circularizes the ligation products. When compared to other methods, RealSeq®-AC provided greatly reduced sequencing bias and allowed the identification of the largest variety of miRNAs in biological samples.
|
| |
|
| Overall design |
The accuracy of detection of miRNAs was evaluated by sequencing miRNA-seq libraries in technical triplicates for each kit by using a synthetic pool of miRNAs (miRXplore pool, Miltenyi Biotec) and also a reference total RNA sample (Human Brain). The coverage of miRNA detection was evaluated by sequencing libraries prepared with TruSeq, RealSeq-AC, NEXTFlex, and QIAseq kits using a Universal miRNA Reference Kit (Agilent).
|
| |
|
| Contributor(s) |
Barberan-Soler S, Kazakov SA |
| Citation(s) |
30173660 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R43 HG007788 |
A method for preparing unbiased miRNA sequencing libraries |
SOMAGENICS INC. |
SERGEI A KAZAKOV |
| R44 HG007788 |
A method for preparing unbiased miRNA sequencing libraries (miR-ACS) |
SOMAGENICS INC. |
SERGEI A KAZAKOV |
|
| |
| Submission date |
Nov 24, 2017 |
| Last update date |
May 16, 2019 |
| Contact name |
Sergio Barberan-Soler |
| E-mail(s) |
[email protected]
|
| Phone |
8314267700
|
| Organization name |
Somagenics Inc
|
| Street address |
2161 Delaware Avenue
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95060 |
| Country |
USA |
| |
|
| Platforms (4)
|
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
| GPL17769 |
Illumina MiSeq (synthetic construct) |
| GPL21697 |
NextSeq 550 (Homo sapiens) |
| GPL22790 |
Illumina MiniSeq (Homo sapiens) |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA419732 |
| SRA |
SRP125586 |
Less...
More...