txid5811[Organism] - GEO DataSets

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Select item 2002834411.

To identify HDAC3 occupancy in the genomes of ME49 and ΔXII-5 strains

(Submitter supplied) Toxoplasma gondii is a ubiquitous protozoan parasite with a complex life cycle containing multiple developmental stages. The parasites have distinct gene expression patterns at different stages to enable stage specific life activities, but the underlying regulatory mechanisms are largely unknown. In this study, we identified a nuclear complex that controls the expression of developmentally regulated genes, especially merozoite specific genes. more...

Organism:: Toxoplasma gondii

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26742
8 Samples

Download data: BIGWIG

Series

Accession:: GSE283441

ID:: 200283441

Select item 2002775502.

A novel nuclear protein complex controlling the expression of developmentally regulated genes in Toxoplasma gondii [RNA-seq]

(Submitter supplied) To investigate the transcriptional effects of TgAP2XII-5 on type II Toxoplasma gondii (ME49) in the tachyzoite and bradyzoite stages. Also, to study the transcriptional role of TgAIP1 on ME49 tachyzoites.

Organism:: Toxoplasma gondii

Type:: Expression profiling by high throughput sequencing

Platform:: GPL33297
18 Samples

Download data: TXT, XLS, XLSX

Series

Accession:: GSE277550

ID:: 200277550

Select item 2002751123.

Toxoplasma gondii transcription factor AP2XII-8 is a key regulator for the G1 phase progression and parasite division

(Submitter supplied) The apicomplexan parasite Toxoplasma gondii can infect humans and almost all warm-blooded animals worldwide, poses significant threat to the public health and of veterinary importance. The acute infection was characterized by fast replication of tachyzoites inside the host cells. During this fast amplification process, the gene expression is highly regulated by series of regulator networks. The G1 phase is usually conserved across species and responsible for the preparation of materials for the next replication cell cycle, but seldom regulators were identified in this stage. more...

Organism:: Toxoplasma gondii

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26742
4 Samples

Download data: BW, NARROWPEAK

Series

Accession:: GSE275112

ID:: 200275112

Select item 2002538854.

In vivo CRISPR screens for Toxoplasma hyperLOPIT-unassigened proteins_2

(Submitter supplied) We performed in vivo CRISPR screens of Toxoplasma targeting hyperLOPIT-unassigened genes during mouse infection.

Organism:: Toxoplasma gondii RH

Type:: Other

Platform:: GPL34114
11 Samples

Download data: TXT, XLSX

Series

Accession:: GSE253885

ID:: 200253885

PubMed

Select item 2002538845.

In vivo CRISPR screens for Toxoplasma hyperLOPIT-unassigened proteins_1

(Submitter supplied) We performed in vivo CRISPR screens of Toxoplasma targeting genes with hyperLOPIT-unassigened subcellular localisation during mouse infection.

Organism:: Toxoplasma gondii RH

Type:: Other

Platform:: GPL34114
12 Samples

Download data: TXT, XLSX

Series

Accession:: GSE253884

ID:: 200253884

PubMed

Select item 2001759196.

A potent HDAC inhibitor blocks Toxoplasma gondii tachyzoite growth and profoundly disrupts parasite gene expression.

(Submitter supplied) Toxoplasmosis is a major health issue worldwide especially for immune-deficient individuals and the offspring of newly infected mothers. It is caused by a unicellular intracellular parasite called Toxoplasma gondii. Although the drugs commonly used to treat toxoplasmosis are efficient, they present serious side effects and adverse events are common. Therefore, there is a need for the discovery of new compounds with potent anti-T. more...

Organism:: Toxoplasma gondii

Type:: Expression profiling by high throughput sequencing

Platform:: GPL23377
6 Samples

Download data: XLSX

Series

Accession:: GSE175919

ID:: 200175919

PubMed Similar studies SRA Run Selector

Select item 2002670147.

Spermine-induced DNA methylation change in human macrophages

(Submitter supplied) Polyamines, crucial molecules involved in cell proliferation and growth, play a pivotal role in cancer development and progression. Within the tumor microenvironment, macrophages, key components of the immune system, exhibit a complex relationship with polyamines. Evidence suggests that polyamines can modulate macrophage polarization, influencing their functional phenotypes. Here, we detected the gene DNA methylation changes in spermine-stimulated human macrophages isolated from PBMCs and TAMs.

Organism:: Leptospira interrogans; Rickettsia typhi; Mycobacterium tuberculosis variant bovis; Mycobacterium tuberculosis; Mycobacterium tuberculosis variant microti; Mycobacterium canetti; Orthohantavirus seoulense; Yersinia enterocolitica; Toxoplasma gondii; Salmonella enterica subsp. enterica serovar Typhimurium; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Campylobacter jejuni; Francisella tularensis subsp. novicida; Yersinia pestis; Staphylococcus aureus; Mycobacterium avium subsp. paratuberculosis; Cowpox virus; Escherichia coli O157:H7; Francisella tularensis subsp. mediasiatica; Paslahepevirus balayani; Yersinia pseudotuberculosis; Rickettsia prowazekii; Bartonella quintana; Mycobacterium avium; Homo sapiens; Streptobacillus moniliformis; Bartonella henselae; Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica

Type:: Methylation profiling by array

Platform:: GPL21445
4 Samples

Download data: IDAT, TXT

Series

Accession:: GSE267014

ID:: 200267014

Select item 2002662048.

Cyclical transcription factor AP2XII-9 is a key activator for asexual division and apicoplast inheritance in Toxoplasma gondii tachyzoite

(Submitter supplied) Toxoplasma gondii is an intracellular parasitic protozoan that poses a significant risk to pregnant women and immunocompromised individuals. T. gondii tachyzoites duplicate rapidly in host cells during acute infection through endodyogeny. This highly regulated division process is accompanied by complex gene regulation networks. TgAP2XII-9 is a cyclical transcription factor, but its specific role in the parasite cell cycle is not fully understood. more...

Organism:: Toxoplasma gondii

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26742
4 Samples

Download data: BW, NARROWPEAK

Series

Accession:: GSE266204

ID:: 200266204

PubMed

Select item 2002636159.

Transcriptome analysis of cytotrophoblasts and syncytiotrophoblasts derived from trophoblast stem cells, mock-infected or infected with Toxoplasma gondii

(Submitter supplied) We tested the relevance of an established stem-cell-derived model of trophoblast development to Toxoplasma gondii infection. Human trophoblast stem cells were cultured under conditions suitable for cytotrophoblast cells or for the differentiation into syncytiotrophoblasts, and subsequently infected with T. gondii. RNA-seq data from both mock-treated and infected cells revealed differences between cell types and their respective responses to T. more...

Organism:: Homo sapiens; Toxoplasma gondii

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL30173 GPL34372
12 Samples

Download data: TXT

Series

Accession:: GSE263615

ID:: 200263615

PubMed Full text in PMC Similar studies

Select item 20024577510.

Translation initiation factor eIF1.2 orchestrates Toxoplasma gondii differentiation by regulating stage-specific gene expression

(Submitter supplied) Toxoplasma gondii persists in humans by converting from actively replicating acute stage tachyzoites to slow-growing chronic stage bradyzoites. The molecular mechanisms that mediate T. gondii differentiation remain poorly understood. Through a chemical mutagenesis screen, we identified translation initiation factor eIF1.2 as being critical for T. gondii differentiation. The presence of an F97L mutation in eIF1.2 identified in the screen or the complete lack eIF1.2 (∆eIF1.2) markedly impeded bradyzoite cyst formation in culture and in the brains of infected mice. more...

Organism:: Toxoplasma gondii

Type:: Expression profiling by high throughput sequencing; Other

Platform:: GPL23466
24 Samples

Download data: TXT

Series

Accession:: GSE245775

ID:: 200245775

PubMed Full text in PMC Similar studies

Select item 20026224111.

Timecourse of transcriptional changes upon eIF4E1 depletion in Toxoplasma gondii

(Submitter supplied) The protozoan parasite Toxoplasma gondii causes serious opportunistic disease due to its ability to persist in patients as latent tissue cysts. The molecular mechanisms coordinating conversion between proliferative parasites (tachyzoites) and latent cysts (bradyzoites) are not fully understood. We previously showed that phosphorylation of eIF2α accompanies bradyzoite formation, suggesting that this clinically relevant process involves regulation of mRNA translation. more...

Organism:: Toxoplasma gondii

Type:: Expression profiling by high throughput sequencing

Platform:: GPL26742
18 Samples

Download data: TABULAR

Series

Accession:: GSE262241

ID:: 200262241

PubMed Full text in PMC Similar studies

Select item 20024844812.

The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony

(Submitter supplied) Commitment to and completion of sexual development are essential for Toxoplasma gondii to produce oocysts in the intestine of the feline host. Understanding of the molecular mechanism responsible for sexual commitment is extremely limited due to the lack of model systems. Here, we show that the transcription factors AP2XI-2 and AP2XII-1 associated with the epigenetic repressors MORC/HDAC3 complex are constitutively expressed in both tachyzoite and bradyzoite stages but not in the merozoite stage. more...

Organism:: Toxoplasma gondii

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26742
6 Samples

Download data: BW

Series

Accession:: GSE248448

ID:: 200248448

PubMed Full text in PMC Similar studies

Select item 20012425113.

Genome-wide binding of the bromdomain protein TgBDP3 in the protozoan parasite Toxoplasma gondii

(Submitter supplied) The bromdomain protein TgBDP3 is associated with the TFIID transcriptioanl initiation complex in Toxoplasma gondii. To determine the genes that are regulated by TgBDP3, the endogenous tgbdp3 locus was modified to introduce a C-terminal HA epitope tag. ChIP-seq was performed on intracellular, replicating tachyzoites expressing TgBDP3-HA. The protein was enriched in the upstream, promoter region of a subset of actively transcribed parasite genes indicating that TgBDP3 has a role in regulation of transcriptional initiation in Toxoplasma.

Organism:: Toxoplasma gondii

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL25973
5 Samples

Download data: BED

Series

Accession:: GSE124251

ID:: 200124251

SRA Run Selector

Select item 20024960414.

AP2XII-1 and AP2XI-2 Suppress Schizogony Gene Expression in Toxoplasma gondii

(Submitter supplied) Toxoplasma gondii is a medically and veterinary important intracellular parasite that undergoes distinct developmental transitions in its intermediate and definitive hosts. The switch between stages of T. gondii is meticulously regulated by a variety of factors. Previous studies have explored the role of the microrchidia (MORC) protein complex as a transcriptional suppressor of sexual commitment. By utilizing immunoprecipitation and mass spectrometry, constituents of this protein complex have been identified, including MORC, Histone Deacetylase 3 (HDAC3), and several ApiApiAP2 transcription factors. more...

Organism:: Toxoplasma gondii

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26742
26 Samples

Download data: BW, TXT

Series

Accession:: GSE249604

ID:: 200249604

PubMed Full text in PMC Similar studies

Select item 20024320715.

Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Toxoplasma gondii

Type:: Other; Expression profiling by high throughput sequencing

Platform:: GPL23466
24 Samples

Download data: BIGWIG, TXT

Series

Accession:: GSE243207

ID:: 200243207

PubMed Full text in PMC Similar studies

Select item 20024320616.

Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii [RIBO-seq]

(Submitter supplied) Ingestion of Toxoplasma gondii results in life-long infection due to its ability to convert from the rapidly disseminating tachyzoite stage to the chronic, encysted bradyzoite stage. The control of mRNA translation has been suggested to play a key role in the signaling required to trigger bradyzoite formation. In eukaryotes, translational control primarily operates at two key points during the assembly of translation initiation factors. more...

Organism:: Toxoplasma gondii

Type:: Other; Expression profiling by high throughput sequencing

Platform:: GPL23466
12 Samples

Download data: TXT

Series

Accession:: GSE243206

ID:: 200243206

PubMed Full text in PMC Similar studies

Select item 20024320317.

Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii [CLIP-seq]

Organism:: Toxoplasma gondii

Type:: Other

Platform:: GPL23466
12 Samples

Download data: BED, BIGWIG

Series

Accession:: GSE243203

ID:: 200243203

PubMed Full text in PMC Similar studies

Select item 20022283518.

In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Toxoplasma gondii

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL23466 GPL26742
24 Samples

Download data: BW

Series

Accession:: GSE222835

ID:: 200222835

PubMed Full text in PMC Similar studies

Select item 20022283219.

In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming [ATAC-seq]

(Submitter supplied) Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. more...