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| Status |
Public on Sep 16, 2023 |
| Title |
In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming [ATAC-seq] |
| Organism |
Toxoplasma gondii |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced in vitro are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3, which in turn restrict the accessibility of chromatin to the transcriptional machinery.
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| Overall design |
We report Tn5 transposase accessiblity data (ATAC-Sequencing) on the RHK80 AP2XII-1 mAID_AP2XI-2 mAID strain in an untreated condition (UT) and induced knockdown of both AP2s (IAA 24h)
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| Contributor(s) |
Antunes AV, Shahinas M, Swale C, Dayana F, Ramakrishnan C, Belmudes L, Cannella D, Corrao C, Couté Y, Hehl AB, Bougdour A, Coppens I, Hakimi MA |
| Citation(s) |
38093015 |
| |
| Submission date |
Jan 13, 2023 |
| Last update date |
Dec 17, 2023 |
| Contact name |
Christopher Swale |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM
|
| Department |
U1209 - Institute of Advanced Biosciences
|
| Lab |
Hakimi Lab
|
| Street address |
Batiment Jean Roger
|
| City |
La Tronche |
| ZIP/Postal code |
38700 |
| Country |
France |
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| Platforms (1) |
| GPL26742 |
Illumina NovaSeq 6000 (Toxoplasma gondii) |
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| Samples (4)
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| GSM6932850 |
AP2-XII-1-KD-HA_AP2-XI-2-KD-Myc_UT_replicate1 |
| GSM6932851 |
AP2-XII-1-KD-HA_AP2-XI-2-KD-Myc_UT_replicate2 |
| GSM6932852 |
AP2-XII-1-KD-HA_AP2-XI-2-KD-Myc_IAA24h_replicate1 |
| GSM6932853 |
AP2-XII-1-KD-HA_AP2-XI-2-KD-Myc_IAA24h_replicate2 |
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| This SubSeries is part of SuperSeries: |
| GSE222835 |
In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming |
|
| Relations |
| BioProject |
PRJNA923619 |
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