GEO DataSets

(Submitter supplied) Durum wheat (Triticum turgidum subsp. durum) is widely grown for pasta production, and more recently, is gaining additional interest due to its resilience to warm, dry climates and its use as an experimental model for wheat research. To enable further research into endosperm development and storage reserve synthesis, we generated a high-quality transcriptomics dataset from developing endosperms of durum variety Kronos, to complement the extensive mutant resources available for this variety. more...

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28728

24 Samples

Download data: TSV

(Submitter supplied) For characterizing the predominant transcriptional profiles associated with spike development progression, and to identify core regulatory candidate genes.

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31178

20 Samples

Download data: TXT

(Submitter supplied) Wheat is one of the most significant crops in terms of human consumption in the world. In a climate change scenario, extreme weather event such as heatwaves will be more frequent especially during the grain-filling (GF) stage and could affect grain weight and quality of crops. Molecular mechanisms underlying the response to short heat stress (HS) have been widely reported for the hexaploid wheat (Triticum aestivum) but the regulatory heat stress mechanisms in tetraploid durum wheat (Triticum turgidum ssp. more...

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20257

6 Samples

Download data: TXT

(Submitter supplied) Heat stress is a major limiting factor for grain yield and grain quality in wheat production. In crops, abiotic stresses have transgenerational effects and the mechanistic basis of stress memory is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Genotype-dependent response patterns to parental and progeny heat stress were found for the leaf physiological traits, harvest components, and grain quality traits measured. more...

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:

GPL20257 GPL28728

24 Samples

Download data: XLSX

(Submitter supplied) Stress events have transgenerational effects that influence plant growth in the subsequent generation. In Mediterranean regions, water-deficit and heat (WH) stress is a frequent issue that negatively affects crop yield and quality. Nitrogen (N) is an essential plant macronutrient and often a yield-limiting factor for crops. Here, the response of durum wheat seedlings to N starvation under the transgenerational effects of WH stress were investigated in two genotypes. more...

Organism:

Triticum turgidum subsp. durum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28728

8 Samples

Download data: XLSX

(Submitter supplied) Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on the parental plants during reproduction could have transgenerational effects on the progenies. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in the progenies. more...

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:

GPL20257 GPL28728

24 Samples

Download data: XLSX

(Submitter supplied) Crop reproduction is highly sensitive to water-deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (T. turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome and degradome in T. more...

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:

GPL28728 GPL20257

134 Samples

Download data: XLSX

(Submitter supplied) Water-deficit and heat stress negatively impact crop production. Mechanisms underlying the response of durum wheat to such stresses are not well understood. With the new durum wheat genome assembly, we conducted the first multi-omics analysis with next-generation sequencing, providing a comprehensive description of the durum wheat small RNAome (sRNAome), mRNA transcriptome, and degradome. Single and combined water-deficit and heat stress were applied to stress-tolerant and -sensitive Australian genotypes to study their response at multiple time-points during reproduction. more...

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:

GPL28728 GPL20257

136 Samples

Download data: XLSX

(Submitter supplied) Water deficiency and heat stress can severely limit crop production and quality. Stress imposed on the parents during reproduction could have transgenerational effects on their progeny. Seeds with different origins can vary significantly in germination time-course and early growth. Here, we investigated how water-deficit and heat stress on parental durum wheat plants affected seedling establishment of the subsequent generation. more...

Organism:

Triticum turgidum subsp. durum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL20257

4 Samples

Download data: XLSX

(Submitter supplied) The phytochromes (PHYs) are a family of photoreceptors which absorb red and far-red light to modulate developmental responses in photosynthetic organisms. We developed knockout mutants of PHYB and PHYC in tetraploid wheat and performed RNA-seq studies to analyze and compare the regulons of each phytochrome under short day photoperiods.

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27807

12 Samples

Download data: TXT

(Submitter supplied) The comparison of transcriptional profiles of roots grown under Fe, S and dual deficiency with those obtained for roots of control plants (grown in a complete nutrient solution) allowed identifying the transcripts differentially expressed in response to each deficiency.

Organism:

Triticum aestivum; Triticum turgidum subsp. durum

Type:

Expression profiling by array

Platform:

GPL13627

12 Samples

Download data: TXT

(Submitter supplied) The phytochromes (PHYs) are a family of photoreceptors which absorb light of red and far-red wavelengths to modulate developmental responses in photosynthetic organisms. We developed knockout mutants of PHYB and PHYC in wheat and performed replicated RNA-seq studies to analyze and compare the regulons of each phytochrome under long day photoperiods.

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19107

32 Samples

Download data: FA, TXT

(Submitter supplied) Durum wheat is an important cereal crop grown mainly in semi-arid environments (e.g. Mediterranean regions) characterized by water scarcity and high temperatures often occurring at the same time. This work reports on a transcriptomic analysis carried out on two durum wheat cultivars (Cappelli and Ofanto) characterized by different water use efficiency (WUE), grown to booting stage and subjected to a combination of drought and heat stresses, a situation similar to the experience of a crop grown in Mediterranean environments and exposed to a terminal heat/drought stress. more...

Organism:

Triticum turgidum subsp. durum; Triticum aestivum

Type:

Expression profiling by array

Platform:

GPL3802

24 Samples

Download data: CEL

(Submitter supplied) The three subgenomes (B, A and D) of common wheat (2n=6x=42) are largely intact, which makes extraction of the BBAA subgenomes as organismally independent genomes possible. Availability of such novel extracted tetraploid wheat (Extracted-tetra) provides a unique opportunity to study whether and to what extent the BBAA subgenomes of common wheat have been irreversibly modified, as well as its attendant biological consequences, during their evolutionary trajectory as allohexaploidy. more...

Organism:

Triticum turgidum subsp. durum; Triticum dicoccoides; Triticum aestivum; Triticum turgidum; Aegilops tauschii; Triticum carthlicum

Type:

Expression profiling by array

Platform:

GPL3802

33 Samples

Download data: CEL

(Submitter supplied) Background: MicroRNAs are endogenous small noncoding RNAs that play critical roles in plant abiotic stress responses. The interaction between miRNA-mRNA targets and their regulatory pathways in response to water deficit stress has been investigated in many plant species. However, the miRNA transcriptome of durum wheat (Triticum turgidum L. ssp. durum) is poorly characterised, with little known about miRNA functions related to water deficit stress. more...

Organism:

Triticum turgidum subsp. durum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL20257

96 Samples

Download data: TAB

(Submitter supplied) Here we use bisulfite conversion of RNA combined with high-throughput IIlumina sequencing (RBS-seq) to identify single-nucleotide resolution of m5C sites in transfer RNAs of all three sub-cellular transcriptomes across six diverse species that include, the single-celled algae Nannochloropsis oculata, the macro algae Caulerpa taxifolia and multi-cellular higher plants Arabidopsis thaliana, Brassica rapa, Triticum durum and Ginkgo biloba.

Organism:

Nannochloropsis oculata; Brassica rapa; Caulerpa taxifolia; Ginkgo biloba; Arabidopsis thaliana; Triticum turgidum subsp. durum

Type:

Other; Non-coding RNA profiling by high throughput sequencing

6 related Platforms

14 Samples

Download data: XLS, XLSX

(Submitter supplied) Here we use bisulfite conversion of RNA combined with high-throughput IIlumina sequencing (RBS-seq) to identify single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs of all three sub-cellular transcriptomes across six diverse species that included, the single-celled algae Nannochloropsis oculata, the macro algae Caulerpa taxifolia and multi-cellular higher plants Arabidopsis thaliana, Brassica rapa, Triticum durum and Ginkgo biloba. more...

Organism:

Nannochloropsis oculata; Ginkgo biloba; Triticum turgidum subsp. durum; Caulerpa taxifolia; Arabidopsis thaliana; Brassica rapa

Type:

Other; Non-coding RNA profiling by high throughput sequencing

6 related Platforms

13 Samples

Download data: XLSX

(Submitter supplied) We profiled genome-wide gene expression changes in newly hybridized triploids (ABD), its genome-duplicated hexaploid (AABBDD), stable synthetic hexaploid (AABBDD) and T. turgidum (AABB) and Ae. tauschii (DD) parental lines of two independent crosses to reconstruct the events of allopolyploidization and genome stabilization.

Organism:

Triticum turgidum subsp. durum; Aegilops tauschii subsp. strangulata; Triticum dicoccon x Aegilops tauschii subsp. strangulata; Triticum turgidum subsp. dicoccum; Triticum aestivum; Triticum turgidum subsp. durum x Aegilops tauschii subsp. strangulata

Type:

Expression profiling by array

Platform:

GPL9805

28 Samples

Download data: TXT

(Submitter supplied) TaGPC1 and TaGPC2 are NAC-domain transcription factors which accelerate the onset of senescence and facilitate nutrient translocation in wheat. We developed knockout mutants of these genes in tetraploid wheat and used RNA-seq to identify the effect of these mutations on the wheat flag leaf transcriptome during monocarpic senescence. Several transporter-related genes were identified which were upregulated during senescence and differentially expressed between genotypes.

Organism:

Triticum turgidum subsp. durum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19107

36 Samples

Download data: GFF, TXT, XLSX

(Submitter supplied) Using 270K Nimblegen Comparative Genomic Hybridization (CGH) array on a set of cv. Chinese Spring deletion lines, a total of 3,671 sequence contigs and scaffolds (~7.8% of chromosome 7B physical length) were mapped into nine deletion bins. In our study we have developed 270K CGH Nimblegen array containing wheat 7B chromosome specific probes and genotyped wheat 7B deletion stocks which have terminal deletions in 7B. more...

Organism:

Triticum turgidum subsp. durum; Triticum aestivum

Type:

Genome variation profiling by genome tiling array

Platform:

GPL18665

22 Samples

Download data: PAIR