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| Status |
Public on Aug 01, 2015 |
| Title |
Expression profiles of allopolyploids and their parents lines during allopolyploidization |
| Platform organism |
Triticum aestivum |
| Sample organisms |
Triticum turgidum subsp. durum; Triticum turgidum subsp. dicoccum; Aegilops tauschii subsp. strangulata; Triticum dicoccon x Aegilops tauschii subsp. strangulata; Triticum turgidum subsp. durum x Aegilops tauschii subsp. strangulata |
| Experiment type |
Expression profiling by array
|
| Summary |
We profiled genome-wide gene expression changes in newly hybridized triploids (ABD), its genome-duplicated hexaploid (AABBDD), stable synthetic hexaploid (AABBDD) and T. turgidum (AABB) and Ae. tauschii (DD) parental lines of two independent crosses to reconstruct the events of allopolyploidization and genome stabilization.
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| Overall design |
Gene expression levels of newly hybridized triploids (ABD), its chromosome-doubled hexaploids (AABBDD), stable synthetic hexaploid (AABBDD) which were selfed during 4 and 13 generations, and their parents, Triticum turgidum (AABB) and Aegilops tauschii (DD) were compared. Total RNA of each line was extracted from three biological replicates of two leaves seedlings except for triploids.For newly hybridized triploids, biological duplicates of two leaves seedlings were used.
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| Contributor(s) |
Jung Y, Kawaura K |
| Citation missing |
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| |
| Submission date |
Jan 12, 2015 |
| Last update date |
Aug 03, 2015 |
| Contact name |
Yeonju Jung |
| E-mail(s) |
[email protected]
|
| Organization name |
Kihara Institute for Biological Research
|
| Department |
Yokohama City University
|
| Lab |
Plant Genome Science
|
| Street address |
Maioka-cho 641-12
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
244-0813 |
| Country |
Japan |
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| Platforms (1) |
| GPL9805 |
Agilent-015520 Custom wheat 38k array |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA272397 |
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