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Series GSE216253 Query DataSets for GSE216253
Status Public on Oct 25, 2022
Title Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
Organism Triticum turgidum subsp. durum
Experiment type Expression profiling by high throughput sequencing
Summary Durum wheat (Triticum turgidum subsp. durum) is widely grown for pasta production, and more recently, is gaining additional interest due to its resilience to warm, dry climates and its use as an experimental model for wheat research. To enable further research into endosperm development and storage reserve synthesis, we generated a high-quality transcriptomics dataset from developing endosperms of durum variety Kronos, to complement the extensive mutant resources available for this variety. Endosperms were dissected from grains harvested at eight timepoints during grain development (6 to 30 days post anthesis (dpa)), then RNA sequencing was used to profile the transcriptome at each stage. The largest changes in gene expression profile were observed between the earlier timepoints, prior to 15 dpa. We detected a total of 29,925 genes that were significantly differentially expressed between at least two timepoints, and clustering analysis revealed nine distinct expression patterns. Overall, we provide a valuable resource for studying endosperm development in this increasingly important crop species.
 
Overall design RNA samples were extracted from endosperm tissue dissected from developing grains. We analysed 8 timepoints (6, 8, 10, 13, 15, 18, 20, 30 days post anthesis) throughout grain development, and three replicates (harvested from three separate plants) per timepoint.
 
Contributor(s) Chen J, Watson-Lazowski A, Vickers M, Seung D
Citation(s) 37460981
Submission date Oct 20, 2022
Last update date Sep 12, 2023
Contact name Martin Vickers
E-mail(s) [email protected]
Organization name John Innes Centre
Street address Colney Ln
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
 
Platforms (1)
GPL28728 Illumina NovaSeq 6000 (Triticum turgidum subsp. durum)
Samples (24)
GSM6664768 S1_6 dpa Rep1
GSM6664769 S2_6 dpa Rep2
GSM6664770 S3_6 dpa Rep3
Relations
BioProject PRJNA892720

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Supplementary file Size Download File type/resource
GSE216253_abundance_est_counts.tsv.gz 3.7 Mb (ftp)(http) TSV
GSE216253_abundance_tpm.tsv.gz 4.9 Mb (ftp)(http) TSV
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Processed data are available on Series record

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