GEO DataSets

Analysis of FACS-sorted CD45-Ter119-Sca-1-CD29+Cxcr4+ skeletal muscle precursor (SMP) cells from young, middle-aged, and old C57BL/6 males. Results provide insight into the molecular mechanisms underlying age-related skeletal muscle and stem cell dysfunction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age sets

Platform:

GPL1261

Series:

GSE50821

14 Samples

Download data: CEL

(Submitter supplied) In this study, we investigated signaling pathways in Skeletal muscle precursors that are altered with aging and age-related deficits in muscle regenerative potential. We performed fluorescence activated cell sorting (FACS) to obtain highly purified skeletal muscle satellite cells from young, middle-aged and old mice. Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting that young blood contains humoral "rejuvenating" factors that can restore regenerative function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4892

Platform:

GPL1261

14 Samples

Download data: CEL

(Submitter supplied) Age-related frailty may in part be due to a decreased competency in skeletal muscle regeneration. The role of the closely related TGFbeta amily molecules myostatin and GDF11 in regeneration is unclear. The commercially available antibody which in a prior report was used to demonstrate an age-related decrease in GDF11 was found to detect both GDF11 and myostatin, and with this reagent it appears that the combination of GDF11 and myostatin increases with age in serum. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

56 Samples

Download data: CEL

(Submitter supplied) We investigated cell-free miRNAs in the blood of young and aged mice. The results indicated marked difference in the level of certain miRNAs between the mice.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL17678

4 Samples

Download data: TXT

(Submitter supplied) ATAC-sequencing of paired Y and O mouse HSCs pre and 3mo post lethal irradiation and HSC transplantation

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21103

16 Samples

Download data: NARROWPEAK

(Submitter supplied) RNA sequencing of hematopoietic stem cells isolated from young (8-12 week) and old (24-27 month) BL6 mice from experimental groups that are intended to describe the transcriptomic consequences of parabiosis on the function of old hematopoietic stem cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

30 Samples

Download data: CSV

(Submitter supplied) Skeletal aging and disease are associated with a misbalance in the opposing actions of osteoblasts and osteoclasts that are responsible for maintaining the integrity of bone tissues. Here, we show through detailed functional and single-cell genomic studies that intrinsic aging of bona fide mouse skeletal stem cells (SSCs) alters bone marrow niche signaling and skews bone and blood lineage differentiation leading to fragile bones that regenerate poorly. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: H5, HTML

(Submitter supplied) Skeletal aging and disease are associated with a misbalance in the opposing actions of osteoblasts and osteoclasts that are responsible for maintaining the integrity of bone tissues. Here, we show through detailed functional and single-cell genomic studies that intrinsic aging of bona fide mouse skeletal stem cells (SSCs) alters bone marrow niche signaling and skews bone and blood lineage differentiation leading to fragile bones that regenerate poorly. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

9 Samples

Download data: XLSX

(Submitter supplied) Skeletal aging and disease are associated with a misbalance in the opposing actions of osteoblasts and osteoclasts that are responsible for maintaining the integrity of bone tissues. Here, we show through detailed functional and single-cell genomic studies that intrinsic aging of bona fide mouse skeletal stem cells (SSCs) alters bone marrow niche signaling and skews bone and blood lineage differentiation leading to fragile bones that regenerate poorly. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

670 Samples

Download data: CSV, HTML

(Submitter supplied) Gene expression profiling using microarray has been limited to profiling of differentially expressed genes at comparison setting since probesets for different genes have different sensitivities. We overcome this limitation by using a very large number of varied microarray datasets as a common reference, so that statistical attributes of each probeset, such as dynamic range or a threshold between low and high expression can be reliably discovered through meta-analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL1261

101 Samples

Download data: CEL, TXT

(Submitter supplied) Heterochronic blood exchange (HBE) has demonstrated that circulating factors restore youthful features to aged tissues. However, the systemic mediators of those rejuvenating effects remain poorly defined. We show that the beneficial effect of young blood on aged muscle regeneration was diminished when serum was depleted of extracellular vesicles (EVs). Whereas EVs from young animals rejuvenate aged cell bioenergetics and skeletal muscle regeneration, aging shifts EV subpopulation heterogeneity and compromises downstream benefits on recipient cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

11 Samples

Download data: TXT

(Submitter supplied) Voluntary exercise enhances old skeletal muscle stem cell (MuSC) function in vivo and ex vivo, dependent on upregulation of Ccnd1. To determine the transcriptional changes associated with these phenotypes, RNA-Seq was performed on MuSCs from young and old mice that had exercised or not exercised, and from young mice with MuSC-specific loss-of-function deletions in zero, one, or both alleles of Ccnd1.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

36 Samples

Download data: TXT

(Submitter supplied) Single-cell RNA sequencing data of Mus Musculus heterochronic parabionts

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

1 Sample

Download data: H5AD

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

996 Samples

Download data: CSV, H5AD, LOOM, TAR

(Submitter supplied) Aging is a complex process involving transcriptomic changes associated with deterioration across multiple tissues and organs, including the brain. Recent studies using heterochronic parabiosis have shown that various aspects of aging-associated decline are modifiable or even reversible. To better understand how this occurs, we performed single-cell transcriptomic profiling of young and old mouse brains following parabiosis. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

56 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL24247

78 Samples

Download data

(Submitter supplied) To gain insight into the mechanisms by which exercise affects the neural stem cell compartment, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of cells from the subventricular zone of the brain of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: RDATA

(Submitter supplied) To gain insight into the mechanisms by which exercise affects the muscle stem cell compartment, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of mononucleated cells from hindlimb muscles of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: RDATA

(Submitter supplied) To gain insight into the mechanisms by which exercise affects hematopoietic stem cells, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of hematopoietic progenitors in the bone marrow of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: RDS

(Submitter supplied) To gain insight into the mechanisms by which exercise affects circulating immune cells, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of circulating immune cells from these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: RDS